The genome of Prunus mume.
The genome of Prunus mume.
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DOI：
10.1038/ncomms2290
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发表时间：
2012
影响因子：
16.6
通讯作者：
中科院分区：
文献类型：
--
作者：
梅花（Prunus mume，mei）是蔷薇科（Rosaceae）李亚科植物中最早进行基因组测序的品种之一，在我国已有3,000多年的栽培历史。在这里，我们通过结合101倍的下一代测序和光学作图数据组装了一个280 M的基因组。我们进一步将锚锚定到8条染色体上，并通过限制性位点相关DNA测序构建遗传图谱。结合梅花基因组数据，成功重建了蔷薇科9条祖先染色体，并描述了3个主要亚科的染色体融合、分裂和复制历史。我们对梅不同组织的转录组进行了测序，并进行了全基因组分析，以揭示梅的特性，包括其在内休眠中的早花调控，对细菌感染的免疫反应和花香的生物合成。梅基因组序列的获得丰富了我们对蔷薇科植物进化的认识，为果树的改良提供了重要数据。 梅花在中国已有3,000多年的驯化历史，是一种重要的观赏植物和水果。在这里，Qixiang Zhang等人通过下一代测序、全基因组作图和限制性位点相关DNA的组合首次组装了其基因组。
Prunus mume (mei), which was domesticated in China more than 3,000 years ago as ornamental plant and fruit, is one of the first genomes among Prunus subfamilies of Rosaceae been sequenced. Here, we assemble a 280M genome by combining 101-fold next-generation sequencing and optical mapping data. We further anchor 83.9% of scaffolds to eight chromosomes with genetic map constructed by restriction-site-associated DNA sequencing. Combining P. mume genome with available data, we succeed in reconstructing nine ancestral chromosomes of Rosaceae family, as well as depicting chromosome fusion, fission and duplication history in three major subfamilies. We sequence the transcriptome of various tissues and perform genome-wide analysis to reveal the characteristics of P. mume, including its regulation of early blooming in endodormancy, immune response against bacterial infection and biosynthesis of flower scent. The P. mume genome sequence adds to our understanding of Rosaceae evolution and provides important data for improvement of fruit trees. The Prunus mume was domesticated in China over 3,000 years ago and is an important ornamental plant and fruit. Here Qixiang Zhang et al. obtain the first assembly of its genome with a combination of next-generation sequencing, whole-genome mapping and restriction-site-associated DNA.
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影响因子：
3.4
作者：
Illa E;Sargent DJ;Lopez Girona E;Bushakra J;Cestaro A;Crowhurst R;Pindo M;Cabrera A;van der Knaap E;Iezzoni A;Gardiner S;Velasco R;Arús P;Chagné D;Troggio M
通讯作者：
Troggio M
影响因子：
5.3
作者：
Jiménez S;Lawton-Rauh AL;Reighard GL;Abbott AG;Bielenberg DG
通讯作者：
Bielenberg DG
影响因子：
4.4
作者：
Chutimanitsakun Y;Nipper RW;Cuesta-Marcos A;Cistué L;Corey A;Filichkina T;Johnson EA;Hayes PM
通讯作者：
Hayes PM
影响因子：
7
作者：
Li, Ruiqiang;Zhu, Hongmei;Wang, Jun
通讯作者：
Wang, Jun
影响因子：
5.1
作者：
Horvath, David P.;Sung, Sibum;Anderson, James
通讯作者：
Anderson, James