The depletion of nuclear glutathione impairs cell proliferation in 3t3 fibroblasts.
The depletion of nuclear glutathione impairs cell proliferation in 3t3 fibroblasts.
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DOI：
10.1371/journal.pone.0006413
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发表时间：
2009-07-29
期刊：
影响因子：
3.7
通讯作者：
Pallardó FV
中科院分区：
文献类型：
--
作者：
Markovic J;Mora NJ;Broseta AM;Gimeno A;de-la-Concepción N;Viña J;Pallardó FV
谷胱甘肽被认为对于哺乳动物细胞和酵母的生存至关重要，但对于原核细胞则不然。谷胱甘肽核库的存在已被证明，但其在细胞增殖和分化中的作用仍然存在争议。我们对 3T3 成纤维细胞的增殖进行了为期 5 天的研究。用两种众所周知的消耗剂马来酸二乙酯 (DEM) 和丁硫氨酸亚砜亚胺 (BSO) 处理细胞，并分别通过分析和共聚焦显微镜技术评估细胞和核谷胱甘肽水平。尽管 BSO 的消耗更持久，但这两种药物都能降低细胞总谷胱甘肽。然而，核谷胱甘肽库可以抵抗 BSO 的消耗，但不能抵抗 DEM 的消耗。有趣的是，DEM 会损害细胞增殖，但 BSO 不会损害细胞增殖。同时用 DEM 和谷胱甘肽乙酯处理细胞以恢复细胞内 GSH 水平，完全阻止了 DEM 对细胞增殖的影响。我们的结果证明了核谷胱甘肽在控制 3T3 成纤维细胞细胞增殖中的重要性，并表明减少的核环境对于细胞在细胞周期中的进展是必要的。
Glutathione is considered essential for survival in mammalian cells and yeast but not in prokaryotic cells. The presence of a nuclear pool of glutathione has been demonstrated but its role in cellular proliferation and differentiation is still a matter of debate. We have studied proliferation of 3T3 fibroblasts for a period of 5 days. Cells were treated with two well known depleting agents, diethyl maleate (DEM) and buthionine sulfoximine (BSO), and the cellular and nuclear glutathione levels were assessed by analytical and confocal microscopic techniques, respectively. Both agents decreased total cellular glutathione although depletion by BSO was more sustained. However, the nuclear glutathione pool resisted depletion by BSO but not with DEM. Interestingly, cell proliferation was impaired by DEM, but not by BSO. Treating the cells simultaneously with DEM and with glutathione ethyl ester to restore intracellular GSH levels completely prevented the effects of DEM on cell proliferation. Our results demonstrate the importance of nuclear glutathione in the control of cell proliferation in 3T3 fibroblasts and suggest that a reduced nuclear environment is necessary for cells to progress in the cell cycle.
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影响因子：
4.1
作者：
BOYLAND, E;CHASSEAUD, LF
通讯作者：
CHASSEAUD, LF
影响因子：
48
作者：
Gutscher, Marcus;Pauleau, Anne-Laure;Dick, Tobias P.
通讯作者：
Dick, Tobias P.
影响因子：
3.8
作者：
BORROZ, KI;BUETLER, TM;EATON, DL
通讯作者：
EATON, DL
影响因子：
4.6
作者：
Conour, JE;Graham, WV;Gaskins, HR
通讯作者：
Gaskins, HR
DOI：
10.1016/0167-4838(89)90047-2
发表时间：
1989-05-01
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
ABEI, M;HARADA, S;OSUGA, T
通讯作者：
OSUGA, T