MicroRNA profiling of pericardial fluid samples from patients with heart failure.
MicroRNA profiling of pericardial fluid samples from patients with heart failure.
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DOI：
10.1371/journal.pone.0119646
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Tavi P
中科院分区：
文献类型：
--
作者：
Kuosmanen SM;Hartikainen J;Hippeläinen M;Kokki H;Levonen AL;Tavi P
多细胞生物通过细胞间通讯维持重要功能。释放细胞外囊泡，将信号传递到远处的靶器官，是实现这种通讯的一种方式。 MicroRNA 也可以从外泌体中的细胞中分泌出来，并充当旁分泌信号分子。此外，microRNA 与包括心血管疾病在内的许多疾病的发病机制有关，并且由于其相对稳定性和易于从临床样本中定量，被认为是有前途的候选生物标志物。心包液含有心脏分泌的激素，已知可以反映心脏功能。在这项研究中，我们试图调查心包液是否含有 microRNA，如果含有，它们是否可以用于区分不同的心血管病理和疾病阶段。心包液是在心脏直视手术期间从心力衰竭患者身上收集的。使用 Exiqon 人类面板 I 和 II 通过定量实时 PCR (qPCR) 测量了总共 51 名患者的 MicroRNA 谱。平均每个样本检测到 256 个 microRNA，每个样本中检测到 742 个已分析的 microRNA 中的 70 个。心包液中最丰富的 5 种 microRNA 是 miR-21-5p、miR-451a、miR-125b-5p、let-7b-5p 和 miR-16-5p。从这些图谱中无法检测到心血管病理学或临床评估的心力衰竭阶段的特定特征，总体而言，尽管研究人群存在异质性，但发现样本的 microRNA 图谱非常相似。测量的 microRNA 谱并没有根据患者的临床特征来分离样本。然而，检测到了一些先前确定的心力衰竭标记物 microRNA。心包液 microRNA 谱似乎是主动和选择性分泌过程的结果，表明 microRNA 可能通过介导心脏细胞之间的局部串扰而充当旁分泌信号因子。
Multicellular organisms maintain vital functions through intercellular communication. Release of extracellular vesicles that carry signals to even distant target organs is one way of accomplishing this communication. MicroRNAs can also be secreted from the cells in exosomes and act as paracrine signalling molecules. In addition, microRNAs have been implicated in the pathogenesis of a large number of diseases, including cardiovascular diseases, and are considered as promising candidate biomarkers due to their relative stability and easy quantification from clinical samples. Pericardial fluid contains hormones secreted by the heart and is known to reflect the cardiac function. In this study, we sought to investigate whether pericardial fluid contains microRNAs and if so, whether they could be used to distinguish between different cardiovascular pathologies and disease stages. Pericardial fluid was collected from heart failure patients during open-heart surgery. MicroRNA profiles of altogether 51 patients were measured by quantitative real-time PCR (qPCR) using Exiqon human panels I and II. On the average, 256 microRNAs were detected per sample, and 70 microRNAs out of 742 profiled microRNAs were detected in every sample. The five most abundant microRNAs in pericardial fluid were miR-21-5p, miR-451a, miR-125b-5p, let-7b-5p and miR-16-5p. No specific signatures for cardiovascular pathologies or clinically assessed heart failure stages could be detected from the profiles and, overall, microRNA profiles of the samples were found to be very similar despite the heterogeneity in the study population. Measured microRNA profiles did not separate the samples according to the clinical features of the patients. However, several previously identified heart failure marker microRNAs were detected. The pericardial fluid microRNA profile appeared to be a result of an active and selective secretory process indicating that microRNAs may act as paracrine signalling factors by mediating the local crosstalk between cardiac cells.
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