Cell Behaviors during Closure of the Choroid Fissure in the Developing Eye.
Cell Behaviors during Closure of the Choroid Fissure in the Developing Eye.
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DOI：
10.3389/fncel.2018.00042
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发表时间：
2018
影响因子：
5.3
通讯作者：
Wilson SW
中科院分区：
文献类型：
--
作者：
Gestri G;Bazin-Lopez N;Scholes C;Wilson SW
缺损是由于脉络膜裂隙融合失败导致的眼部形态发生的缺陷。它是人类最常见的先天性缺陷之一，会严重影响视力。然而，对调控脉络膜裂隙闭合的细胞机制知之甚少。利用斑马鱼视杯的高分辨率共聚焦成像，我们发现在融合过程中，在远端近端和从内到外梯度的裂隙内排列的细胞中，尖-基极性被重建。这一过程伴随着细胞的增殖、血管的移位以及脉络膜裂隙和眼周间充质(POM)之间的接触。为了研究POM细胞在缝隙闭合中的作用，我们将视泡移植到卵黄上，使它们在POM耗尽的情况下发育。异位眼通常会形成脉络膜裂，但在这种情况下，尽管视网膜的鼻唇和颞唇适时地贴合，但融合失败。这项研究解决了脉络膜裂隙融合的一些细胞行为，并支持POM在脉络膜裂隙融合中的作用。
Coloboma is a defect in the morphogenesis of the eye that is a consequence of failure of choroid fissure fusion. It is among the most common congenital defects in humans and can significantly impact vision. However, very little is known about the cellular mechanisms that regulate choroid fissure closure. Using high-resolution confocal imaging of the zebrafish optic cup, we find that apico-basal polarity is re-modeled in cells lining the fissure in proximal to distal and inner to outer gradients during fusion. This process is accompanied by cell proliferation, displacement of vasculature, and contact between cells lining the choroid fissure and periocular mesenchyme (POM). To investigate the role of POM cells in closure of the fissure, we transplanted optic vesicles onto the yolk, allowing them to develop in a situation where they are depleted of POM. The choroid fissure forms normally in ectopic eyes but fusion fails in this condition, despite timely apposition of the nasal and temporal lips of the retina. This study resolves some of the cell behaviors underlying choroid fissure fusion and supports a role for POM in choroid fissure fusion.
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影响因子：
--
作者：
GEERAETS, R
通讯作者：
GEERAETS, R
DOI：
10.1002/jez.b.21189
发表时间：
2007-09-15
影响因子：
2.2
作者：
Hoffman, Trevor L.;Javier, Anna L.;Schilling, Thomas F.
通讯作者：
Schilling, Thomas F.
影响因子：
16.2
作者：
Cavodeassi F;Carreira-Barbosa F;Young RM;Concha ML;Allende ML;Houart C;Tada M;Wilson SW
通讯作者：
Wilson SW
影响因子：
2.5
作者：
Cechmanek, Paula Bernice;McFarlane, Sarah
通讯作者：
McFarlane, Sarah
影响因子：
11.8
作者：
Burstyn-Cohen, T;Kalcheim, C
通讯作者：
Kalcheim, C