Implant surface culture may be a useful adjunct to standard tissue sampling culture for identification of pathogens accounting for fracture-device-related infection: a within-person randomized agreement study of 42 patients.
Implant surface culture may be a useful adjunct to standard tissue sampling culture for identification of pathogens accounting for fracture-device-related infection: a within-person randomized agreement study of 42 patients.
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DOI：
10.2340/17453674.2022.4530
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发表时间：
2022-09-07
影响因子：
3.7
通讯作者：
中科院分区：
文献类型：
--
作者：
由于标准组织取样培养（TSC）的阳性率仍不令人满意，导致植入器械相关感染（FDRI）的病原体鉴定一直是一个挑战。本研究评价了植入物表面培养（ISC）作为标准TSC的辅助手段用于鉴定FDRI相关微生物的有效性。在2020年11月至2022年3月期间，诊断为国际骨折相关感染（FRI）共识组定义的FDRI并适用于植入物取出的患者接受了两种细菌检测方法。ISC和TSC的测试顺序是随机选择的每例患者，作为一个人内随机设计。对于ISC，用生理盐水冲洗两次后，用胰蛋白酶大豆琼脂轻轻覆盖回收的植入物，然后在37℃ 5% CO2下孵育长达14天。TSC采集5份标本送广州南方医科大学南方医院检验科进行培养鉴定。纳入了42例连续患者，平均年龄为46岁。最常见的感染部位和植入物类型分别为胫骨（21例）和带螺钉的钢板（30例）。两种方法共发现21例阳性结果，鉴定的病原体一致。ISC发现另外15名患者显示阳性结果，TSC为阴性。此外，ISC的平均培养时间短于TSC（1.5天对3.2天）。ISC可作为TSC的一种辅助手段，用于检测FDRI致病菌，其阳性率较高，培养时间较短。
Identification of pathogens causing fracture-device-related infection (FDRI) is always a challenge as the positive rate of standard tissue sampling culture (TSC) remains unsatisfactory. This study evaluates the efficiency of implant surface culture (ISC) as an adjunct to standard TSC for identification of FDRI-associated microorganisms. Between November 2020 and March 2022, patients diagnosed with FDRI defined by the International Fracture-Related Infection (FRI) Consensus Group, and indicated for implant removal, underwent both methods for bacteria detection. The test order of ISC and TSC was randomly selected for each patient included, as a withinperson randomized design. For ISC, the recovered implants were gently covered with tryptic soy agar after rinsing with normal saline twice, and then incubated at 37℃ 5% CO2 for up to 14 days. For TSC, 5 specimens were sampled and sent to the Clinical Laboratory of Southern Medical University Nanfang Hospital, Guangzhou, for culture and identification. 42 consecutive patients were included, with a mean age of 46 years. The most frequent infection site and implant type were the tibia (21 cases) and plates with screws (30 cases), respectively. Altogether 21 patients were found with positive outcomes by both methods, and the identified pathogens were consistent. ISC found an additional 15 patients showing positive results, which were negative by TSC. Furthermore, the mean culture time of ISC was shorter than that of TSC (1.5 days vs. 3.2 days). ISC may be a useful adjunct to TSC for detection of bacteria causing FDRI, with a relatively higher positive rate and a shorter culture time.
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影响因子：
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作者：
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影响因子：
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影响因子：
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DOI：
10.1136/bmj.j2835
发表时间：
2017-06-30
期刊：
BMJ (Clinical research ed.)
影响因子：
--
作者：
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通讯作者：
Altman DG