Comparative analysis of plant genomes allows the definition of the "Phytolongins": a novel non-SNARE longin domain protein family.
Comparative analysis of plant genomes allows the definition of the "Phytolongins": a novel non-SNARE longin domain protein family.
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DOI：
10.1186/1471-2164-10-510
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发表时间：
2009-11-04
期刊：
影响因子：
4.4
通讯作者：
Filippini F
中科院分区：
文献类型：
--
作者：
Vedovato M;Rossi V;Dacks JB;Filippini F
亚细胞运输是真核细胞的标志。由于它们在这个过程中的关键作用，SNARE蛋白受到了极大的关注。然而，大多数R-SNARE或"长蛋白"也具有高度保守的N-末端折叠。已知该"longin结构域"在调节SNARE活性和通过与其他运输蛋白相互作用靶向中发挥多种作用。然而，在真核生物中的Longins的多样性和互补性知之甚少。我们的比较基因组调查确定了一个新的长蛋白相关蛋白质家族，被称为“植物长蛋白”，因为它们是陆地植物特有的。Phytolongins与longins共享N-末端longin结构域和C-末端跨膜结构域;然而，在中心区域，SNARE基序被新区域取代。系统发育分析指出植物长苷是植物特异性VAMP72长苷亚家族的衍生物，并允许阐明植物长苷的进化。"Longins"已被定义为由Longin结构域和SNARE基序两者组成的R-SNARE。然而，表达的基因同种型和剪接变体的长蛋白是非陷阱基序含有长蛋白的例子。植物长蛋白是一个非SNARE长蛋白结构域蛋白家族，以及最近关于参与囊泡融合的蛋白质中长蛋白样折叠的保守性的证据，（例如Trs20系链）和囊泡形成（例如σ和μ adaptin）强调了长蛋白样结构域在蛋白质运输中的重要性，并表明它是真核细胞膜的原始构建模块之一-贩卖机器
Subcellular trafficking is a hallmark of eukaryotic cells. Because of their pivotal role in the process, a great deal of attention has been paid to the SNARE proteins. Most R-SNAREs, or "longins", however, also possess a highly conserved, N-terminal fold. This "longin domain" is known to play multiple roles in regulating SNARE activity and targeting via interaction with other trafficking proteins. However, the diversity and complement of longins in eukaryotes is poorly understood. Our comparative genome survey identified a novel family of longin-related proteins, dubbed the "Phytolongins" because they are specific to land plants. Phytolongins share with longins the N-terminal longin domain and the C-terminal transmembrane domain; however, in the central region, the SNARE motif is replaced by a novel region. Phylogenetic analysis pinpoints the Phytolongins as a derivative of the plant specific VAMP72 longin sub-family and allows elucidation of Phytolongin evolution. "Longins" have been defined as R-SNAREs composed of both a longin domain and a SNARE motif. However, expressed gene isoforms and splice variants of longins are examples of non-SNARE motif containing longins. The discovery of Phytolongins, a family of non-SNARE longin domain proteins, together with recent evidence on the conservation of the longin-like fold in proteins involved in both vesicle fusion (e.g. the Trs20 tether) and vesicle formation (e.g. σ and μ adaptin) highlight the importance of the longin-like domain in protein trafficking and suggest that it was one of the primordial building blocks of the eukaryotic membrane-trafficking machinery.
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影响因子：
4
作者：
Dacks, Joel B.;Field, Mark C.
通讯作者：
Field, Mark C.
影响因子：
2.2
作者：
Adl, SM;Simpson, AGB;Taylor, MFJR
通讯作者：
Taylor, MFJR
影响因子：
4.8
作者：
Jang, SB;Kim, YG;Oh, BH
通讯作者：
Oh, BH
影响因子：
13.8
作者：
Filippini, F;Rossi, V;D'Esposito, M
通讯作者：
D'Esposito, M
DOI：
10.1093/protein/10.6.673
发表时间：
1997-06-01
期刊：
PROTEIN ENGINEERING
影响因子：
--
作者：
Cserzo, M;Wallin, E;Elofsson, A
通讯作者：
Elofsson, A