Molecular Cloning, Expression Pattern and Genotypic Effects on Glucoraphanin Biosynthetic Related Genes in Chinese Kale (Brassica oleracea var. alboglabra Bailey).
Molecular Cloning, Expression Pattern and Genotypic Effects on Glucoraphanin Biosynthetic Related Genes in Chinese Kale (Brassica oleracea var. alboglabra Bailey).
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羽衣甘蓝萝卜硫苷生物合成相关基因的分子克隆、表达模式及基因型效应
DOI：
10.3390/molecules201119688
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发表时间：
2015-11-11
期刊：
影响因子：
--
通讯作者：
Lei J
中科院分区：
文献类型：
--
作者：
Yin L;Chen C;Chen G;Cao B;Lei J
葡萄糖苷是一种植物次生代谢物，参与植物防御并赋予十字花科蔬菜促进健康的特性。本研究从芥蓝（Brassica oleracea var. alboglabra Bailey）中克隆了3个参与葡萄糖苷代谢的基因——支链氨基转移酶4 （BCAT4）、甲基硫代烷基苹果酸合成酶1 （MAM1）和二同甲硫氨酸n -羟化酶（CYP79F1）。序列同源性和系统发育分析鉴定了这些基因，证实了芥蓝的进化地位。BCAT4、MAM1和CYP79F1转录本在子叶、叶和茎中的表达水平高于花和叶柄。BCAT4、MAM1和CYP79F1在整个叶片发育过程中均有表达，在幼苗期表达水平较低。不同品种间葡萄糖苷含量差异较大，干重为0.25 ~ 2.73µmol·g−1 DW。BCAT4和MAM1在营养-生殖过渡期高表达，而CYP79F1在生殖期高表达。在高糖苷含量基因型中，BCAT4、MAM1和CYP79F1的表达量显著升高。这些结果为进一步了解BCAT4、MAM1和CYP79F1基因在芥蓝萝卜硫苷合成中的作用提供了依据。
Glucoraphanin is a plant secondary metabolite that is involved in plant defense and imparts health-promoting properties to cruciferous vegetables. In this study, three genes involved in glucoraphanin metabolism, branched-chain aminotransferase 4 (BCAT4), methylthioalkylmalate synthase 1 (MAM1) and dihomomethionine N-hydroxylase (CYP79F1), were cloned from Chinese kale (Brassica oleracea var. alboglabra Bailey). Sequence homology and phylogenetic analysis identified these genes and confirmed the evolutionary status of Chinese kale. The transcript levels of BCAT4, MAM1 and CYP79F1 were higher in cotyledon, leaf and stem compared with flower and silique. BCAT4, MAM1 and CYP79F1 were expressed throughout leaf development with lower transcript levels during the younger stages. Glucoraphanin content varied extensively among different varieties, which ranged from 0.25 to 2.73 µmol·g−1 DW (dry weight). Expression levels of BCAT4 and MAM1 were high at vegetative–reproductive transition phase, while CYP79F1 was expressed high at reproductive phase. BCAT4, MAM1 and CYP79F1 were expressed significantly high in genotypes with high glucoraphanin content. All the results provided a better understanding of the roles of BCAT4, MAM1 and CYP79F1 in the glucoraphanin biosynthesis of Chinese kale.
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影响因子：
14.9
作者：
Geu-Flores, Fernando;Nour-Eldin, Hussam H.;Nielsen, Morten T.;Halkier, Barbara A.
通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
6
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