Phytophthora capsici sterol reductase PcDHCR7 has a role in mycelium development and pathogenicity.
Phytophthora capsici sterol reductase PcDHCR7 has a role in mycelium development and pathogenicity.
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DOI：
10.1098/rsob.210282
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发表时间：
2022-04
期刊：
影响因子：
5.8
通讯作者：
Liu X
中科院分区：
文献类型：
--
作者：
Wang W;Zhang F;Zhang S;Xue Z;Xie L;Govers F;Liu X
甾醇的从头生物合成对于大多数真核生物是至关重要的;然而，一些生物缺乏这一途径，包括大多数卵菌。疫霉属是甾醇营养缺陷型的，但值得注意的是，保留了一些编码甾醇生物合成途径中的酶的基因。在这里，我们表明，PcDHCR 7，辣椒疫霉中预测编码Δ7-甾醇还原酶的基因，显示出多种功能。在酿酒酵母中表达后，PcDHCR 7具有Δ7-甾醇还原酶活性。在辣椒疫霉中敲除PcDHCR 7导致将麦角固醇转化为菜籽固醇的能力丧失，这意味着PcDHCR 7在辣椒疫霉本身中具有Δ7-固醇还原酶活性。这使得辣椒疫霉能够转化从环境中募集的甾醇，以更好地利用。将Δ PcDHCR 7转化子与野生型菌株及互补型菌株的生物学特性进行比较，结果表明PcDHCR 7在菌丝发育和游动孢子致病性中起关键作用。进一步的转录组分析表明，Δ PcDHCR 7基因组中许多基因的表达发生了变化，这些基因参与了不同的生物学过程。辣椒疫霉有可能通过重塑其转录组来补偿PcDHCR 7缺失引起的缺陷。
The de novo biosynthesis of sterols is critical for the majority of eukaryotes; however, some organisms lack this pathway, including most oomycetes. Phytophthora spp. are sterol auxotrophic but, remarkably, have retained a few genes encoding enzymes in the sterol biosynthesis pathway. Here, we show that PcDHCR7, a gene in Phytophthora capsici predicted to encode Δ7-sterol reductase, displays multiple functions. When expressed in Saccharomyces cerevisiae, PcDHCR7 showed the Δ7-sterol reductase activity. Knocking out PcDHCR7 in P. capsici resulted in loss of the capacity to transform ergosterol into brassicasterol, which means PcDHCR7 has the Δ7-sterol reductase activity in P. capsici itself. This enables P. capsici to transform sterols recruited from the environment for better use. The biological characteristics of ΔPcDHCR7 transformants were compared with those of the wild-type strain and a PcDHCR7 complemented transformant, and the results showed that PcDHCR7 plays a key role in mycelium development and pathogenicity of zoospores. Further analysis of the transcriptome indicated that the expression of many genes changed in the ΔPcDHCR7 transformant, which involve in different biological processes. It is possible that P. capsici compensates for the defects caused by the loss of PcDHCR7 by remodelling its transcriptome.
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影响因子：
14.8
作者：
Gietz, R. Daniel;Schiestl, Robert H.
通讯作者：
Schiestl, Robert H.
疫霉甲基化组由 6mA 甲基转移酶调节并与适应性基因组区域相关
影响因子：
12.3
作者：
Chen H;Shu H;Wang L;Zhang F;Li X;Ochola SO;Mao F;Ma H;Ye W;Gu T;Jiang L;Wu Y;Wang Y;Kamoun S;Dong S
通讯作者：
Dong S
DOI：
10.1093/bioinformatics/8.3.275
发表时间：
1992-06-01
期刊：
COMPUTER APPLICATIONS IN THE BIOSCIENCES
影响因子：
--
作者：
JONES, DT;TAYLOR, WR;THORNTON, JM
通讯作者：
THORNTON, JM
影响因子：
--
作者：
Jee, HJ;Ko, WH
通讯作者：
Ko, WH
影响因子：
7.2
作者：
Griebel, Thomas;Zeier, Juergen
通讯作者：
Zeier, Juergen