Human mitochondrial RNA decay mediated by PNPase-hSuv3 complex takes place in distinct foci.
Human mitochondrial RNA decay mediated by PNPase-hSuv3 complex takes place in distinct foci.
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DOI：
10.1093/nar/gks1130
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发表时间：
2013-01
影响因子：
14.9
通讯作者：
Szczesny RJ
中科院分区：
文献类型：
--
作者：
Borowski LS;Dziembowski A;Hejnowicz MS;Stepien PP;Szczesny RJ
RNA降解通常由蛋白质复合物介导，并且可以发生在特定的病灶中，例如真核生物细胞质中的P体。在人类线粒体中，对RNA衰变机制的空间组织一无所知，负责RNA降解的核糖核酸酶也没有被鉴定出来。我们证明，沉默的人多核苷酸磷酸化酶（PNIPs）的原因积累的RNA衰变中间体，并增加了线粒体转录的半衰期。荧光寿命成像显微镜与Förster共振能量转移和双分子荧光互补（BiFC）实验相结合，证明PNIPs和hSuv3解旋酶（Suv3，hSuv3p和SUPV3L1）在人体线粒体中体内形成RNA降解复合物。这种复合物，称为降解体，仅在特定的焦点（称为D焦点）中形成，其与线粒体RNA和类核共定位。值得注意的是，PNIPs和hSuv3之间的相互作用是有效的线粒体RNA降解所必需的。这提供了间接的证据，降解体依赖的线粒体RNA衰变发生在病灶。
RNA decay is usually mediated by protein complexes and can occur in specific foci such as P-bodies in the cytoplasm of eukaryotes. In human mitochondria nothing is known about the spatial organization of the RNA decay machinery, and the ribonuclease responsible for RNA degradation has not been identified. We demonstrate that silencing of human polynucleotide phosphorylase (PNPase) causes accumulation of RNA decay intermediates and increases the half-life of mitochondrial transcripts. A combination of fluorescence lifetime imaging microscopy with Förster resonance energy transfer and bimolecular fluorescence complementation (BiFC) experiments prove that PNPase and hSuv3 helicase (Suv3, hSuv3p and SUPV3L1) form the RNA-degrading complex in vivo in human mitochondria. This complex, referred to as the degradosome, is formed only in specific foci (named D-foci), which co-localize with mitochondrial RNA and nucleoids. Notably, interaction between PNPase and hSuv3 is essential for efficient mitochondrial RNA degradation. This provides indirect evidence that degradosome-dependent mitochondrial RNA decay takes place in foci.
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DOI：
10.1016/j.bbagrm.2011.12.005
发表时间：
2012-09
影响因子：
4.7
作者：
Kasiviswanathan, Rajesh;Collins, Tammy R. L.;Copeland, William C.
通讯作者：
Copeland, William C.
影响因子：
64.5
作者：
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通讯作者：
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影响因子：
5.4
作者：
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通讯作者：
Cook, Peter R.
影响因子：
16
作者：
He, F;Li, XR;Jacobson, A
通讯作者：
Jacobson, A
影响因子：
2.5
作者：
GOLIK, P;SZCZEPANEK, T;LAZOWSKA, J
通讯作者：
LAZOWSKA, J