Additive and transcript-specific effects of KPAP1 and TbRND activities on 3' non-encoded tail characteristics and mRNA stability in Trypanosoma brucei.
Additive and transcript-specific effects of KPAP1 and TbRND activities on 3' non-encoded tail characteristics and mRNA stability in Trypanosoma brucei.
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DOI：
10.1371/journal.pone.0037639
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Read LK
中科院分区：
文献类型：
--
作者：
Zimmer SL;McEvoy SM;Menon S;Read LK
Short, non-encoded oligo(A), oligo(U), or A/U tails can impact mRNA stability in kinetoplastid mitochondria. However, a comprehensive picture of the relative effects of these modifications in RNA stability is lacking. Furthermore, while the U-preferring exoribonuclease TbRND acts on U-tailed gRNAs, its role in decay of uridylated mRNAs has only been cursorily investigated. Here, we analyzed the roles of mRNA 3′ tail composition and TbRND in RNA decay using cells harbouring single or double knockdown of TbRND and the KPAP1 poly(A) polymerase. Analysis of mRNA abundance and tail composition reveals dramatic and transcript-specific effects of adenylation and uridylation on mitochondrial RNAs. Oligo(A) and A-rich tails can stabilize a proportion of edited and never-edited RNAs. However, non-tailed RNAs are not inherently unstable, implicating additional stability determinants and/or spatial segregation of sub-populations of a given RNA in regulation of RNA decay. Oligo(U) tails, which have been shown to contribute to decay of some never-edited RNAs, are not universally destabilizing. We also show that RNAs display very different susceptibility to uridylation in the absence of KPAP1, a factor that may contribute to regulation of decay. Finally, 3′ tail composition apparently impacts the ability of an RNA to be edited.
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影响因子：
3.4
作者：
Panigrahi, Aswini K.;Ogata, Yuko;Zikova, Alena;Anupama, Atashi;Dalley, Rachel A.;Acestor, Nathalie;Myler, Peter J.;Stuart, Kenneth D.
通讯作者：
Stuart, Kenneth D.
影响因子：
4.5
作者：
Panigrahi, Aswini K.;Ernst, Nancy Lewis;Stuart, Kenneth D.
通讯作者：
Stuart, Kenneth D.
影响因子：
4.5
作者：
Hashimi, Hassan;Zikova, Alena;Lukes, Julius
通讯作者：
Lukes, Julius
影响因子：
--
作者：
Penschow, JL;Sleve, DA;Read, LK
通讯作者：
Read, LK
影响因子：
2.9
作者：
CHOMCZYNSKI, P;SACCHI, N
通讯作者：
SACCHI, N