A comprehensive analysis of Trypanosoma brucei mitochondrial proteome.
A comprehensive analysis of Trypanosoma brucei mitochondrial proteome.
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DOI：
10.1002/pmic.200800477
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发表时间：
2009-01
期刊：
影响因子：
3.4
通讯作者：
Stuart, Kenneth D.
中科院分区：
文献类型：
--
作者：
Panigrahi, Aswini K.;Ogata, Yuko;Zikova, Alena;Anupama, Atashi;Dalley, Rachel A.;Acestor, Nathalie;Myler, Peter J.;Stuart, Kenneth D.
用质谱仪(2D-LC-MS/MS和Gel-LC-MS/MS)分析了布氏毛滴虫大而单一的线粒体的组成。共鉴定出2897种蛋白质，代表了原环状细胞蛋白质组的相当大比例，这证实了绝大多数基因预测的有效性。数据还显示，被注释为假想(物种特有)的基因被过度预测，几乎所有被注释为假想的、不太可能的基因都没有表达。通过将质谱学数据与基因组序列进行比较，发现了40个以前没有预测到的基因。这些数据放在一个公开可用的基于网络的数据库中(www.TrypsProteome.org)。线粒体总蛋白质组估计为1,008个蛋白质，其中401个、196个和283个蛋白质分别以高、中和低的置信度分配给线粒体。剩余的线粒体蛋白通过统计方法估计，尽管不能进行个别分配。已识别的蛋白质预测了在大分子、代谢、能量产生和运输过程中的作用，提供了布鲁氏毛滴虫线粒体的蛋白质含量和功能的全面概况。
The composition of the large, single, mitochondrion of T. brucei was characterized by mass spectrometry (2D-LC-MS/MS and gel-LC-MS/MS) analyses. A total of 2,897 proteins representing a substantial proportion of procyclic form cellular proteome were identified, which confirmed the validity of the vast majority of gene predictions. The data also showed that the genes annotated as hypothetical (species specific) were over-predicted and that virtually all genes annotated as hypothetical, unlikely are not expressed. By comparing the mass spectrometry data with genome sequence, 40 genes were identified that were not previously predicted. The data are placed in a publicly available web-based database (www.TrypsProteome.org). The total mitochondrial proteome is estimated at 1,008 proteins, with 401, 196, and 283 assigned to the mitochondrion with high, moderate, and lower confidence, respectively. The remaining mitochondrial proteins were estimated by statistical methods although individual assignments could not be made. The identified proteins have predicted roles in macromolecular, metabolic, energy generating, and transport processes providing a comprehensive profile of the protein content and function of the T. brucei mitochondrion.
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影响因子：
4.5
作者：
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通讯作者：
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