Nicotinic acetylcholine receptor (CHRN) expression and function in cultured human adult fungiform (HBO) taste cells.
Nicotinic acetylcholine receptor (CHRN) expression and function in cultured human adult fungiform (HBO) taste cells.
复制标题
DOI：
10.1371/journal.pone.0194089
复制
发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Lyall V
中科院分区：
文献类型：
--
作者：
Qian J;Mummalaneni S;Larsen J;Grider JR;Spielman AI;Özdener MH;Lyall V
在啮齿类动物中，CHRN参与尼古丁和乙醇的苦味转导。目前，尚不清楚CHRN是否在人类味觉细胞中表达，以及它们是否在转导尼古丁和乙醇的苦味或在神经体液肽的合成和释放中发挥作用。因此，我们研究了CHRN在HBO细胞中的表达和功能作用。使用分子技术，我们证明了HBO细胞的一个子集表达CHRN，也共表达TRPM 5，T1 R3或T2 R38。暴露HBO细胞尼古丁或乙醇急性或尼古丁慢性诱导的差异增加的表达CHRN mRNA和蛋白质的剂量和时间依赖性的方式。急性暴露HBO细胞含有尼古丁加乙醇的混合物诱导相对于尼古丁或乙醇单独治疗的CHRN mRNA的增加较小。HBO细胞的一个子集响应于尼古丁，乙酰胆碱和ATP与[Ca 2 +]i的瞬时增加。尼古丁对[Ca ~（2+）]i的影响是对美加明敏感的。ELISA法检测HBO细胞中脑源性神经营养因子（BDNF）蛋白的表达。急性尼古丁暴露减少了HBO细胞中的BDNF，并增加了培养基中BDNF的释放。RT-PCR检测HEK 293细胞中CHRNs的表达。与HBO细胞不同，CHRN定位于大多数HEK 293细胞中，并且大多数HEK 293细胞对尼古丁和乙醇刺激有反应，[Ca 2 +]i瞬时增加。HEK 293细胞中的BDNF水平显著高于HBO细胞，但尼古丁诱导的培养基中BDNF的释放是BDNF细胞内容物的一部分。我们的结论是，CHRN表达TRPM 5阳性HBO细胞。暴露于尼古丁和乙醇以剂量和时间依赖性方式调节CHRN mRNA表达。尼古丁诱导HBO细胞合成和释放BDNF。
In rodents, CHRNs are involved in bitter taste transduction of nicotine and ethanol. Currently, it is not clear if CHRNs are expressed in human taste cells and if they play a role in transducing the bitter taste of nicotine and ethanol or in the synthesis and release of neurohumoral peptides. Accordingly, we investigated the expression and functional role of CHRNs in HBO cells. Using molecular techniques, we demonstrate that a subset of HBO cells express CHRNs that also co-express TRPM5, T1R3 or T2R38. Exposing HBO cells to nicotine or ethanol acutely or to nicotine chronically induced a differential increase in the expression of CHRN mRNA and protein in a dose- and time-dependent manner. Acutely exposing HBO cells to a mixture containing nicotine plus ethanol induced a smaller increase in CHRN mRNAs relative to nicotine or ethanol treatment alone. A subset of HBO cells responded to nicotine, acetylcholine and ATP with a transient increase in [Ca2+]i. Nicotine effects on [Ca2+]i were mecamylamine sensitive. Brain-derived neurotrophic factor (BDNF) protein was detected in HBO cells using ELISA. Acute nicotine exposure decreased BDNF in HBO cells and increased BDNF release in the medium. CHRNs were also detected in HEK293 cells by RT-PCR. Unlike HBO cells, CHRNs were localized in most of HEK293 cells and majority of HEK293 cells responded to nicotine and ethanol stimulation with a transient increase in [Ca2+]i. BDNF levels in HEK293 cells were significantly higher than in HBO cells but the nicotine induced release of BDNF in the media was a fraction of the BDNF cellular content. We conclude that CHRNs are expressed in TRPM5 positive HBO cells. CHRN mRNA expression is modulated by exposure to nicotine and ethanol in a dose- and time-dependent manner. Nicotine induces the synthesis and release of BDNF in HBO cells.
登录
查看更多内容
影响因子：
4.2
作者：
HENNINGFIELD, JE;STAPLETON, JM;LONDON, ED
通讯作者：
LONDON, ED
影响因子：
3.3
作者：
De Rosa, MJ;Esandi, MD;Bouzat, C
通讯作者：
Bouzat, C
影响因子：
3.5
作者：
Al-Qudah M;Alkahtani R;Akbarali HI;Murthy KS;Grider JR
通讯作者：
Grider JR
影响因子：
3.7
作者：
Chatterjee S;Santos N;Holgate J;Haass-Koffler CL;Hopf FW;Kharazia V;Lester H;Bonci A;Bartlett SE
通讯作者：
Bartlett SE
DOI：
10.1016/j.alcohol.2009.09.027
发表时间：
2009-12
期刊：
Alcohol (Fayetteville, N.Y.)
影响因子：
--
作者：
Bell RL;Eiler BJ 2nd;Cook JB;Rahman S
通讯作者：
Rahman S