A Chemical Method for Specific Capture of Circulating Tumor Cells Using Label-Free Polyphenol-Functionalized Films
A Chemical Method for Specific Capture of Circulating Tumor Cells Using Label-Free Polyphenol-Functionalized Films
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使用无标记多酚官能化薄膜特异性捕获循环肿瘤细胞的化学方法
DOI：
10.1021/acs.chemmater.8b01646
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发表时间：
2018-06
影响因子：
8.6
通讯作者：
Jia Lingyun
中科院分区：
文献类型：
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作者：
Yang Liwei;Sun He;Jiang Wenning;Xu Ting;Song Bing;Peng Ruilian;Han Lulu;Jia Lingyun
循环肿瘤细胞（CTC）的捕获主要依赖于肿瘤细胞与外周血单个核细胞（PBMC）之间的生物亲和力和物理性质的差异，但仅使用化学方法有效捕获CTC仍然很少见。受多酚类化合物优异的抗炎作用的启发，我们使用多酚鞣酸官能化（TA官能化）膜来开发一种简单而有效的化学策略，用于CTC的无标记捕获。该膜可以通过金属-多酚配位相互作用在几分钟内容易地制备，同时巧妙地结合了排斥PBMC粘附和增强癌细胞粘附的效果。TA的没食子酰基被发现在癌细胞对膜的增强粘附中起着至关重要的作用。由于TA膜对癌细胞的偏好超过PBMC，因此该膜可以在没有捕获抗体的帮助下有效地捕获广泛的癌细胞（产率>76%）。重要的是，Viabil…
The capture of circulating tumor cells (CTCs) mainly depends on the biological affinity and differences in physical properties between tumor cells and peripheral blood mononuclear cells (PBMCs), but the effective capture of CTCs using only a chemical method is still rare. Inspired by the excellent anti-inflammatory effects of polyphenols, we used polyphenol tannic acid-functionalized (TA-functionalized) film to develop a simple and yet effective chemical strategy for label-free capture of CTCs. The film could be easily prepared within minutes by metal–polyphenol coordination interaction while tactfully integrating the effects of repelling PBMC adhesion and enhancing cancer cell adhesion. The galloyl groups of TA were found to play a crucial role in the enhanced adhesion of cancer cells to the film. As a result of the preference of TA film for cancer cells over PBMCs, the film could effectively capture a broad range of cancer cells without the aid of capture antibodies (yield >76%). Importantly, the viabil...
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影响因子：
16.6
作者：
Wang, Shutao;Wang, Hao;Jiao, Jing;Chen, Kuan-Ju;Owens, Gwen E.;Kamei, Ken-ichiro;Sun, Jing;Sherman, David J.;Behrenbruch, Christian P.;Wu, Hong;Tseng, Hsian-Rong
通讯作者：
Tseng, Hsian-Rong
影响因子：
14.2
作者：
Kawai, K;Tsuno, NH;Nagawa, H
通讯作者：
Nagawa, H
影响因子：
16.6
作者：
Myung, Ja Hye;Gajjar, Khyati A.;Saric, Jelena;Eddington, David T.;Hong, Seungpyo
通讯作者：
Hong, Seungpyo
影响因子：
17.1
作者：
Yoon, Hyeun Joong;Kozminsky, Molly;Nagrath, Sunitha
通讯作者：
Nagrath, Sunitha
影响因子：
38.3
作者：
通讯作者：
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