Comparative metagenomic and metatranscriptomic analyses of microbial communities in acid mine drainage.
Comparative metagenomic and metatranscriptomic analyses of microbial communities in acid mine drainage.
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酸性矿山排水中微生物群落的比较宏基因组和宏转录组分析
DOI：
10.1038/ismej.2014.245
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发表时间：
2015-07
期刊：
影响因子：
--
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中科院分区：
文献类型：
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The microbial communities in acid mine drainage have been extensively studied to reveal their roles in acid generation and adaption to this environment. Lacking, however, are integrated community- and organism-wide comparative gene transcriptional analyses that could reveal the response and adaptation mechanisms of these extraordinary microorganisms to different environmental conditions. In this study, comparative metagenomics and metatranscriptomics were performed on microbial assemblages collected from four geochemically distinct acid mine drainage (AMD) sites. Taxonomic analysis uncovered unexpectedly high microbial biodiversity of these extremely acidophilic communities, and the abundant taxa ofAcidithiobacillus,LeptospirillumandAcidiphiliumexhibited high transcriptional activities. Community-wide comparative analyses clearly showed that the AMD microorganisms adapted to the different environmental conditions via regulating the expression of genes involved in multiplein situfunctional activities, including low-pH adaptation, carbon, nitrogen and phosphate assimilation, energy generation, environmental stress resistance, and other functions. Organism-wide comparative analyses of the active taxa revealed environment-dependent gene transcriptional profiles, especially the distinct strategies used byAcidithiobacillus ferrivoransandLeptospirillum ferrodiazotrophumin nutrients assimilation and energy generation for survival under different conditions. Overall, these findings demonstrate that the gene transcriptional profiles of AMD microorganisms are closely related to the site physiochemical characteristics, providing clues into the microbial response and adaptation mechanisms in the oligotrophic, extremely acidic environments.
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影响因子：
2.9
作者：
Hallberg, Kevin B.;Gonzalez-Toril, Elena;Johnson, D. Barrie
通讯作者：
Johnson, D. Barrie
影响因子：
4.4
作者：
Goltsman, Daniela S. Aliaga;Denef, Vincent J.;Banfield, Jillian F.
通讯作者：
Banfield, Jillian F.
影响因子：
5.1
作者：
Guazzaroni, Maria-Eugenia;Morgante, Veronica;Gonzalez-Pastor, Jose E.
通讯作者：
Gonzalez-Pastor, Jose E.
影响因子：
14.9
作者：
Cole JR;Wang Q;Cardenas E;Fish J;Chai B;Farris RJ;Kulam-Syed-Mohideen AS;McGarrell DM;Marsh T;Garrity GM;Tiedje JM
通讯作者：
Tiedje JM
影响因子：
14.9
作者：
Franceschini A;Szklarczyk D;Frankild S;Kuhn M;Simonovic M;Roth A;Lin J;Minguez P;Bork P;von Mering C;Jensen LJ
通讯作者：
Jensen LJ