Intraperitoneal injection of 17β‐estradiol increases ovarian smad2/3 expression in Yangtze sturgeon Acipenser dabryanus
Intraperitoneal injection of 17β‐estradiol increases ovarian smad2/3 expression in Yangtze sturgeon Acipenser dabryanus
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腹腔注射17β雌二醇增加长江鲟卵巢smad2/3表达
DOI：
10.1111/are.15817
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发表时间：
2022-03
影响因子：
2
通讯作者：
Chuangju Li
中科院分区：
文献类型：
--
作者：
Huamei Yue;Huan Ye;Rui Ruan;Hao Du;Qiwei Wei;Chuangju Li
Smad蛋白是tgf - β（转化生长因子β）通路中涉及的下游信号因子。本研究旨在揭示雌二醇对濒危鲟鱼大鲟Smad信号的反馈调节。在扬子鲟中鉴定出两个受体激活（R‐）smad同源物smad2和smad3，全长cDNA序列分别为1824和1876个碱基对，分别编码467和426个氨基酸。多序列比对表明，Smad2和Smad3在脊椎动物中具有高度保守性。系统发育分析表明，Smad2基因在长江中华鲟中比Smad3基因更为保守。组织分布分析显示，smad2和smad3在心脏、肝脏、脾脏、肾脏、肠道、睾丸、卵巢、中脑和垂体中普遍存在转录。腹腔注射17β‐雌二醇（E2）后，卵巢中smad2和smad3的转录水平显著升高（p< 0.05）。E2组芳香化酶（cyp19a1a）和雌激素受体β (erβ)的转录均降低（p< 0.05）。这些结果表明，E2可能通过下调鲟鱼卵巢中的芳香化酶和ERβ，对R - smad信号传导产生正反馈作用。
Smad (mothers against decapentaplegic homologue) proteins are the downstream signalling factors involved in TGFβ (transforming growth factor beta) pathways. The present study was aimed to reveal the estradiol feedback regulation of Smad signalling in one of the endangered sturgeon speciesAcipenser dabyanus. Two receptor‐activated (R‐) smad paralogues,smad2 and smad3were identified in Yangtze sturgeon, whose full‐length cDNA sequences were 1,824 and 1,876 base pairs, encoding 467 and 426 amino acids respectively. Multiple sequence alignment suggested that both Smad2 and Smad3 were highly conserved among vertebrates. Phylogenetic analysis indicated that Smad2 was more conserved than Smad3 in Yangtze sturgeon. Tissue distribution analysis revealed that bothsmad2andsmad3transcribed ubiquitously in heart, liver, spleen, kidney, intestine, testis, ovary, midbrain and pituitary. Transcription levels ofsmad2andsmad3in the ovary were increased significantly after 17β‐estradiol (E2) intraperitoneal injection (p< 0.05). Besides, transcriptions of aromatase (cyp19a1a), and oestrogen receptor beta (erβ) were decreased due to E2 injection (p< 0.05). These results indicate that E2 exerts positive feedback effect on R‐smad signalling, potentially through the downregulation of aromatase and ERβ, in the ovary of sturgeons.
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影响因子：
5.3
作者：
Wicks, SJ;Lui, S;Chantry, A
通讯作者：
Chantry, A
影响因子：
4.8
作者：
Matsuda, T;Yamamoto, T;Saatcioglu, F
通讯作者：
Saatcioglu, F
DOI：
10.1083/jcb.200106023
发表时间：
2001-12-10
期刊：
The Journal of cell biology
影响因子：
--
作者：
Hanyu A;Ishidou Y;Ebisawa T;Shimanuki T;Imamura T;Miyazono K
通讯作者：
Miyazono K
影响因子：
4
作者：
A. Moustakas;S. Souchelnytskyi;C. Heldin
通讯作者：
A. Moustakas;S. Souchelnytskyi;C. Heldin
影响因子：
8.7
作者：
Jian Xu;J. Oakley;E. Mcgee
通讯作者：
Jian Xu;J. Oakley;E. Mcgee