Quantification of age-related changes of α-tocopherol in lysosomal membranes in murine tissues and human fibroblasts.
Quantification of age-related changes of α-tocopherol in lysosomal membranes in murine tissues and human fibroblasts.
复制标题
鼠组织和人成纤维细胞溶酶体膜中α-生育酚与年龄相关变化的定量
作者：
König J;Besoke F;Stuetz W;Malarski A;Jahreis G;Grune T;Höhn A.
考虑到α‐生育酚（α‐Toc）作为抗氧化应激的有效保护因子的生物学功能，这种抗氧化剂是衰老研究的重点。为了了解α - Toc在衰老过程中的作用，我们研究了补充RRR - α - Toc后，年轻和老年原代人成纤维细胞中α - Toc的浓度。此外，我们测定了10周、18个月和24个月大的小鼠的大脑、肾脏和肝脏组织中α‐Toc的含量，这些小鼠被喂食含有100 mg/kg dl‐α‐生育酚乙酸酯的标准饲料。我们还分析了分离溶酶体中α‐Toc的浓度以及α‐Toc转运蛋白Niemann Pick C1 （NPC1）、Niemann Pick C2 （NPC2）和脂蛋白脂肪酶的表达。获得的数据显示，小鼠肝脏、肾脏和脑组织以及人类真皮成纤维细胞中α - Toc的显著增加与年龄相关。肝脏和肾脏溶酶体的α‐Toc含量也随着年龄的增长而升高。成年和老年小鼠肾组织中NPC1和NPC2蛋白含量显著降低。衰老的人真皮成纤维细胞也显示出NPC1的减少。年轻和年老成纤维细胞的补充也导致NPC1含量下降，表明该蛋白在α‐Toc分布中起直接作用。我们的研究结果表明，在不同的小鼠组织和人类成纤维细胞中，α - Toc呈年龄依赖性增加。此外，α - Toc的饱和和细胞内分布似乎强烈依赖于这种维生素的可用性以及溶酶体蛋白NPC1的存在。©2016 BioFactors, 42(3): 307-315, 2016
Considering the biological function of α‐tocopherol (α‐Toc) as a potent protective factor against oxidative stress, this antioxidant is in the focus of aging research. To understand the role of α‐Toc during aging we investigated α‐Toc concentrations in young and aged primary human fibroblasts after supplementation with RRR‐α‐Toc. Additionally, α‐Toc contents were determined in brain, kidney, and liver tissue of 10 week‐, 18 month‐, and 24 month‐old mice, which were fed a standard diet containing 100 mg/kg dl‐α‐tocopheryl acetate. α‐Toc concentrations in isolated lysosomes and the expression of the α‐Toc transport proteins Niemann Pick C1 (NPC1), Niemann Pick C2 (NPC2), and lipoprotein lipase were also analyzed. Obtained data show a significant age‐related increase of α‐Toc in murine liver, kidney, and brain tissue as well as in human dermal fibroblasts. Also liver and kidney lysosomes are marked by elevated α‐Toc contents with aging. NPC1 and NPC2 protein amounts are significantly decreased in adult and aged murine kidney tissue. Also aged human dermal fibroblasts show decreased NPC1 amounts. Supplementation of young and aged fibroblasts led also to decreased NPC1 amounts, suggesting a direct role of this protein in α‐Toc distribution. Our results indicate an age‐dependent increase of α‐Toc in different murine tissues as well as in human fibroblasts. Furthermore saturation and intracellular distribution of α‐Toc seem to be strongly dependent on the availability of this vitamin as well as on the presence of the lysosomal protein NPC1. © 2016 BioFactors, 42(3):307–315, 2016
登录
查看更多内容
维生素 C 和维生素 E 对氧化 DNA 损伤的影响：随机对照试验的结果。
DOI：
--
发表时间：
2000
期刊：
Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology
影响因子：
--
作者：
Huang,HY;Helzlsouer,KJ;Appel,LJ
通讯作者：
Appel,LJ
维生素 E 贩运
影响因子：
5.2
作者：
M. Traber;G. Burton;R. Hamilton
通讯作者：
R. Hamilton
大鼠膳食 RRR-α- 和全 rac-α-生育酚之间的组织区别
DOI：
--
发表时间：
1991
期刊：
影响因子：
--
作者：
W. Behrens;R. Madère
通讯作者：
R. Madère
妊娠和疟疾感染期间 DDT 残留与血浆视黄醇、α-生育酚和 β-胡萝卜素的关系：泰国北部克伦族妇女的病例对照研究
影响因子：
9.8
作者：
W. Stuetz;R. McGready;Thein Cho;T. Prapamontol;H. Biesalski;K. Stȩpniewska;F. Nosten
通讯作者：
F. Nosten
血浆番茄红素浓度的年龄相关变化与脂肪量相关，但维生素 E 的变化无关
影响因子：
3.6
作者：
P. Grolier;Y. Boirie;E. Levadoux;M. Brandolini;P. Borel;V. Azais;B. Beaufrère;P. Ritz
通讯作者：
P. Ritz