Modeling chemotherapy induced peripheral neuropathy (CIPN) in vitro: Prospects and limitations.
Modeling chemotherapy induced peripheral neuropathy (CIPN) in vitro: Prospects and limitations.
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DOI：
10.1016/j.expneurol.2019.113140
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发表时间：
2020-04
影响因子：
5.3
通讯作者：
Hoke A
中科院分区：
文献类型：
--
作者：
Lehmann HC;Staff NP;Hoke A
神经细胞培养已成为研究化疗药物毒性病理机制和开发神经保护方法的重要工具。它们提供了剖析疾病机制和分子途径的机会，同时允许精确控制各种物理化学环境的混杂因素。因此，每年都有越来越多的体外研究发表，以破译紫杉烷、长春花碱、蛋白酶体抑制剂和铂衍生物的神经毒性机制和/或测试神经保护策略。在这里，我们提供了过去或目前用于体外模拟化疗诱导神经病变的细胞培养技术和结果测量的综述。此外，我们讨论了它们的优点以及它们的局限性，以及如何提高中毒性神经病变领域细胞培养研究的效率和可重复性。
Neuronal cell cultures have been used as an essential tool for studying pathomechanisms of toxicity of chemotherapeutic drugs and to develop neuroprotective approaches. They offer the opportunity to dissect disease mechanisms and molecular pathways while allowing precise control of a variety of confounding factors of the physio-chemical environment. As such, a growing number of in vitro studies are published each year to decipher mechanisms of neurotoxicity of taxanes, vinca alcaloids, proteasome inhibitors and platin derivatives and/or to test neuroprotective strategies. Here, we provide a review of cell culture techniques and outcome measures that have been used in the past or are currently employed to model chemotherapy induced neuropathy in vitro. Furthermore, we discuss their advantages as well as their limitations and ways to enhance efficiency and reproducibility of cell culture studies in the field of toxic neuropathy.
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影响因子：
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Dottori M