Evaluation of gene, protein and neurotrophin expression in the brain of mice exposed to space environment for 91 days.
Evaluation of gene, protein and neurotrophin expression in the brain of mice exposed to space environment for 91 days.
复制标题
DOI：
10.1371/journal.pone.0040112
复制
发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Ohira Y
中科院分区：
文献类型：
--
作者：
Santucci D;Kawano F;Ohira T;Terada M;Nakai N;Francia N;Alleva E;Aloe L;Ochiai T;Cancedda R;Goto K;Ohira Y
研究了3个月微重力环境暴露对小鼠脑组织基因和蛋白表达的影响。此外，神经生物学参数，神经生长因子（NGF）和脑源性神经营养因子（BDNF）的反应，也在小脑，海马，皮质和肾上腺进行了评价。观察到基因和蛋白质表达的航天相关变化。上调基因的生物学过程与免疫反应、代谢过程和/或炎症反应有关。细胞成分的变化涉及微粒体和囊泡分数也注意到。分子功能类别与各种酶的活性有关。下调基因的生物学过程与多种代谢和分解代谢过程有关。细胞成分与胞质和胞核有关。下调的分子功能与催化和氧化还原酶活性有关。与地面对照相比，在太空飞行后观察到28种蛋白质的上调。这些蛋白质与线粒体代谢、ATP合成和水解、钙/钙调素代谢、神经系统、蛋白质和/或氨基酸转运有关。下调的蛋白质与线粒体代谢有关。野生型动物的海马、皮质和肾上腺中NGF的表达在航天后有下降的趋势。对于多效生长因子转基因小鼠，与航天飞行相关的NGF减少仅发生在肾上腺。在BDNF对航天飞行的反应中，没有观察到大脑和肾上腺各部分之间的一致趋势。虽然暴露于真实的微重力影响了大脑中许多基因和蛋白质的表达，这些基因和蛋白质已被证明与广泛的生物功能有关，但仍然不清楚大脑的功能特性如何受到3个月暴露于微重力的影响。
Effects of 3-month exposure to microgravity environment on the expression of genes and proteins in mouse brain were studied. Moreover, responses of neurobiological parameters, nerve growth factor (NGF) and brain derived neurotrophic factor (BDNF), were also evaluated in the cerebellum, hippocampus, cortex, and adrenal glands. Spaceflight-related changes in gene and protein expression were observed. Biological processes of the up-regulated genes were related to the immune response, metabolic process, and/or inflammatory response. Changes of cellular components involving in microsome and vesicular fraction were also noted. Molecular function categories were related to various enzyme activities. The biological processes in the down-regulated genes were related to various metabolic and catabolic processes. Cellular components were related to cytoplasm and mitochondrion. The down-regulated molecular functions were related to catalytic and oxidoreductase activities. Up-regulation of 28 proteins was seen following spaceflight vs. those in ground control. These proteins were related to mitochondrial metabolism, synthesis and hydrolysis of ATP, calcium/calmodulin metabolism, nervous system, and transport of proteins and/or amino acids. Down-regulated proteins were related to mitochondrial metabolism. Expression of NGF in hippocampus, cortex, and adrenal gland of wild type animal tended to decrease following spaceflight. As for pleiotrophin transgenic mice, spaceflight-related reduction of NGF occured only in adrenal gland. Consistent trends between various portions of brain and adrenal gland were not observed in the responses of BDNF to spaceflight. Although exposure to real microgravity influenced the expression of a number of genes and proteins in the brain that have been shown to be involved in a wide spectrum of biological function, it is still unclear how the functional properties of brain were influenced by 3-month exposure to microgravity.
登录
查看更多内容
影响因子：
2
作者：
Frigeri, Antonio;Iacobas, Dumitru A.;Iacobas, Sanda;Nicchia, Grazia Paola;Desaphy, Jean Francois;Camerino, Diana Conte;Svelto, Maria;Spray, David C.
通讯作者：
Spray, David C.
影响因子：
3.7
作者：
DeFelipe, J;Arellano, JI;Llinás, R
通讯作者：
Llinás, R
影响因子：
64.8
作者：
LINDSAY, RM;HARMAR, AJ
通讯作者：
HARMAR, AJ
DOI：
10.1073/pnas.83.16.6184
发表时间：
1986-08-01
影响因子：
11.1
作者：
ALOE, L;ALLEVA, E;LEVIMONTALCINI, R
通讯作者：
LEVIMONTALCINI, R
影响因子：
3.3
作者：
Ishihara, A;Ohira, Y;Edgerton, VR
通讯作者：
Edgerton, VR