A novel member of the netrin family, beta-netrin, shares homology with the beta chain of laminin: identification, expression, and functional characterization.
A novel member of the netrin family, beta-netrin, shares homology with the beta chain of laminin: identification, expression, and functional characterization.
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Netrin家族的一个新成员Beta-Netrin与层粘连蛋白的β链共享同源性：识别，表达和功能表征。
DOI：
10.1083/jcb.151.2.221
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发表时间：
2000-10-16
期刊：
影响因子：
--
通讯作者：
Burgeson RE
中科院分区：
文献类型：
--
作者：
Koch M;Murrell JR;Hunter DD;Olson PF;Jin W;Keene DR;Brunken WJ;Burgeson RE
netrin是层粘连蛋白相关分子的家族。在这里，我们描述了一个新的家庭成员，β-netrin。β-Netrin与层粘连蛋白链短臂的NH 2末端同源;它含有层粘连蛋白样结构域VI和3.5个层粘连蛋白EGF重复序列以及netrin C结构域。与其他netrin不同，这种新netrin与层粘连蛋白β链更相关，因此，它的名称为β-netrin。组织分布的初步分析显示，肾脏、心脏、卵巢、视网膜和嗅球是高表达的组织。我们已经在真核细胞表达系统中表达了该分子，并制备了表达产物的抗体。原位杂交和免疫组织化学均用于描述β-netrin的细胞来源和β-netrin的沉积位置。β-Netrin是一种基底膜成分;它存在于血管系统、肾脏和卵巢的基底膜中。此外，β-netrin在脑内的一组有限的纤维束中表达，包括外侧嗅束和犁鼻神经。进行了功能研究，并显示β-netrin促进嗅球外植体的神经突伸长。总之，这些数据表明β-netrin在神经、肾脏和血管发育中是重要的。
The netrins are a family of laminin-related molecules. Here, we characterize a new member of the family, β-netrin. β-Netrin is homologous to the NH2 terminus of laminin chain short arms; it contains a laminin-like domain VI and 3.5 laminin EGF repeats and a netrin C domain. Unlike other netrins, this new netrin is more related to the laminin β chains, thus, its name β-netrin. An initial analysis of the tissue distribution revealed that kidney, heart, ovary, retina, and the olfactory bulb were tissues of high expression. We have expressed the molecule in a eukaryotic cell expression system and made antibodies to the expressed product. Both in situ hybridization and immunohistochemistry were used to describe the cellular source of β-netrin and where β-netrin is deposited. β-Netrin is a basement membrane component; it is present in the basement membranes of the vasculature, kidney, and ovaries. In addition, β-netrin is expressed in a limited set of fiber tracts within the brain, including the lateral olfactory tract and the vomeronasal nerve. Functional studies were performed and show that β-netrin promotes neurite elongation from olfactory bulb explants. Together, these data suggest that β-netrin is important in neural, kidney, and vascular development.
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影响因子：
16.2
作者：
ISHII, N;WADSWORTH, WG;HEDGECOCK, EM
通讯作者：
HEDGECOCK, EM
影响因子：
16.2
作者：
Deiner, MS;Kennedy, TE;Sretavan, DW
通讯作者：
Sretavan, DW
影响因子：
56.9
作者：
Kim, S;Wadsworth, WG
通讯作者：
Wadsworth, WG
影响因子：
64.5
作者：
KeinoMasu, K;Masu, M;TessierLavigne, M
通讯作者：
TessierLavigne, M
影响因子：
4.8
作者：
Amano, S;Scott, IC;Burgeson, RE
通讯作者：
Burgeson, RE