Peroxidase activities of gold nanowires synthesized by TMV as template and their application in detection of cancer cells
Peroxidase activities of gold nanowires synthesized by TMV as template and their application in detection of cancer cells
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TMV为模板合成金纳米线的过氧化物酶活性及其在癌细胞检测中的应用
DOI：
10.1007/s00253-020-10520-3
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发表时间：
2020-03
影响因子：
5
通讯作者：
Zhao Min
中科院分区：
文献类型：
--
作者：
Qu Yuejun;Yang Yue;Du Renjie;Zhao Min
报道了一种结合Au、烟草花叶病毒(TMV)和叶酸的传感方法，用于基于过氧化物酶样活性的选择性、灵敏和比色检测肿瘤细胞。以TMV为模板合成了高深宽比的金纳米线。得到了直径为4 nm、长度为200~300 nm的Au@TMV纳米线(AT)络合物。此外，由于TMV具有生物相容性，表面有许多氨基和羧基，AT被叶酸偶联，形成了叶酸(FA)偶联的AT复合材料(ATF)，并通过FTIR进行了测试。此外，还研究了类过氧化物酶的性质，优化了模拟酶活力的最佳条件。最后，HeLa和其他肿瘤细胞在表面表达过量的叶酸受体，可以与叶酸特异性结合。作为ATF与HeLa细胞的特异性结合，ATF的过氧化物酶特性被用于检测癌细胞(方案1)。癌细胞的检测不仅是定性的，而且是定量的。在这项研究中，使用目前的方法可以检测到低至2000个癌细胞/毫升。
A sensing methodology that combines Au, tobacco mosaic virus (TMV), and folic acid for selective, sensitive, and colorimetric detection of tumor cells based on the peroxidase-like activity was reported in this study. Gold nanowires with a high aspect ratio were synthesized using TMV as a template. Au@TMV nanowire (AT) complex was obtained with diameter of 4 nm and length between 200 and 300 nm. In addition, since TMV was biocompatible and had many amino and carboxyl groups on its surface, AT was conjugated by folate to form a folic acid (FA)–conjugated AT composite (ATF) and tested by FTIR measurements. Furthermore, the peroxidase-like properties were studied and the optimal conditions for mimic enzyme activity were optimized. Finally, HeLa and other tumor cells expressed excessive receptors of folate on the surface, which can specifically bind to folic acid. As the specific binding of ATF with HeLa cells, the peroxidase properties of ATF were used for detection of cancer cells (Scheme 1). The cancer cells were detected not only qualitatively but also quantitatively. In this study, as low as 2000 cancer cells/mL could be detected using the current method.
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影响因子：
17.1
作者：
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通讯作者：
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影响因子：
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影响因子：
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作者：
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通讯作者：
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影响因子：
3.8
作者：
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通讯作者：
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影响因子：
3.2
作者：
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