Tissue expression and developmental regulation of chicken cathelicidin antimicrobial peptides.
Tissue expression and developmental regulation of chicken cathelicidin antimicrobial peptides.
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DOI：
10.1186/2049-1891-3-15
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发表时间：
2012-05-31
影响因子：
7
通讯作者：
Zhang G
中科院分区：
文献类型：
--
作者：
Achanta M;Sunkara LT;Dai G;Bommineni YR;Jiang W;Zhang G
Cathelicidins是脊椎动物中存在的具有强效杀微生物和免疫调节活性的抗微生物肽的主要家族。已在鸡中鉴定出四种cathelicidin，即fowlicidin 1至3和cathelicidin B1。作为了解它们在鸡的早期先天宿主防御中的作用的第一步，我们研究了所有四种cathelicidins的组织和发育表达模式。实时荧光定量PCR显示，在整个胃肠道，呼吸道和泌尿生殖道，以及在所有的主要和次要免疫器官的鸡的四个cathelicidins的丰富表达。杀鸡素1 ~ 3的组织表达模式相似，在骨髓和肺中表达最高，而cathelicidin B1在法氏囊中合成最丰富。此外，组织特异性的监管模式是显而易见的，所有四个cathelicidins在孵化后的第一个28天。的表达fowlicidins 1至3表现出年龄依赖性的增加，在盲肠扁桃体和肺，而所有四个cathelicidins在孵化后第4天达到峰值，在法氏囊中，与逐渐下降的第28天。在第28天，在盲肠中也观察到鸡抗菌素1至3的表达的突然增加，而在第14天，在肺和盲肠扁桃体中观察到凯萨林菌素B1的最高表达。总的来说，cathelicidins在广泛的组织中的存在及其在发育过程中的大量增强表达表明其在鸡的早期宿主防御和抗病性中的潜在重要作用。
Cathelicidins are a major family of antimicrobial peptides present in vertebrate animals with potent microbicidal and immunomodulatory activities. Four cathelicidins, namely fowlicidins 1 to 3 and cathelicidin B1, have been identified in chickens. As a first step to understand their role in early innate host defense of chickens, we examined the tissue and developmental expression patterns of all four cathelicidins. Real-time PCR revealed an abundant expression of four cathelicidins throughout the gastrointestinal, respiratory, and urogenital tracts as well as in all primary and secondary immune organs of chickens. Fowlicidins 1 to 3 exhibited a similar tissue expression pattern with the highest expression in the bone marrow and lung, while cathelicidin B1 was synthesized most abundantly in the bursa of Fabricius. Additionally, a tissue-specific regulatory pattern was evident for all four cathelicidins during the first 28 days after hatching. The expression of fowlicidins 1 to 3 showed an age-dependent increase both in the cecal tonsil and lung, whereas all four cathelicidins were peaked in the bursa on day 4 after hatching, with a gradual decline by day 28. An abrupt augmentation in the expression of fowlicidins 1 to 3 was also observed in the cecum on day 28, while the highest expression of cathelicidin B1 was seen in both the lung and cecal tonsil on day 14. Collectively, the presence of cathelicidins in a broad range of tissues and their largely enhanced expression during development are suggestive of their potential important role in early host defense and disease resistance of chickens.
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影响因子：
4.8
作者：
Yenugu, S;Hamil, KG;Hall, SH
通讯作者：
Hall, SH
影响因子：
3.1
作者：
Hasenstein, Jason R.;Zhang, Guolong;Lamont, Susan J.
通讯作者：
Lamont, Susan J.
影响因子：
3.7
作者：
Sunkara LT;Achanta M;Schreiber NB;Bommineni YR;Dai G;Jiang W;Lamont S;Lillehoj HS;Beker A;Teeter RG;Zhang G
通讯作者：
Zhang G
影响因子：
5.4
作者：
Bommineni, Yugendar R.;Dai, Huaien;Zhang, Guolong
通讯作者：
Zhang, Guolong
影响因子：
3.6
作者：
Van Dijk, Albert;Tersteeg-Zijderveld, Monique H. G.;Haagsman, Henk P.
通讯作者：
Haagsman, Henk P.