Butyrate enhances disease resistance of chickens by inducing antimicrobial host defense peptide gene expression.
Butyrate enhances disease resistance of chickens by inducing antimicrobial host defense peptide gene expression.
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DOI：
10.1371/journal.pone.0027225
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Zhang G
中科院分区：
文献类型：
--
作者：
Sunkara LT;Achanta M;Schreiber NB;Bommineni YR;Dai G;Jiang W;Lamont S;Lillehoj HS;Beker A;Teeter RG;Zhang G
宿主防御肽(HDPs)是一大类天然的广谱抗菌剂，是几乎所有生命形式的重要免疫第一线。内源性HDPs合成的特异性增强可能代表着一种很有前途的抗生素替代疾病控制方法。在这项研究中，我们测试了外源给予丁酸盐的假说，丁酸盐是细菌发酵未消化的膳食纤维所产生的一种主要类型的短链脂肪酸，能够诱导HDP并增强鸡的抗病能力。我们已经发现，丁酸盐是HD11巨噬细胞以及原代单核细胞、骨髓细胞、空肠和盲肠外植体中几种(但不是全部)鸡HDP的有效诱导剂。此外，丁酸盐处理提高了鸡单核细胞对肠炎沙门氏菌的抗菌活性，对炎性细胞因子的产生、吞噬功能和细胞的氧化爆发能力的影响最小。此外，饲料中添加0.1%丁酸盐显著提高了HDP基因在鸡肠道中的表达。更重要的是，在实验感染肠炎沙门氏菌后，这种喂养策略导致盲肠内的细菌滴度降低了近10倍。总之，这些结果表明，丁酸诱导的内源性HDP的合成是哺乳动物和禽类共有的一种遗传保守的固有宿主防御机制，饲料中添加丁酸有可能进一步发展成为一种方便的抗生素替代策略，以增强宿主的天然免疫和抗病能力。
Host defense peptides (HDPs) constitute a large group of natural broad-spectrum antimicrobials and an important first line of immunity in virtually all forms of life. Specific augmentation of synthesis of endogenous HDPs may represent a promising antibiotic-alternative approach to disease control. In this study, we tested the hypothesis that exogenous administration of butyrate, a major type of short-chain fatty acids derived from bacterial fermentation of undigested dietary fiber, is capable of inducing HDPs and enhancing disease resistance in chickens. We have found that butyrate is a potent inducer of several, but not all, chicken HDPs in HD11 macrophages as well as in primary monocytes, bone marrow cells, and jejuna and cecal explants. In addition, butyrate treatment enhanced the antibacterial activity of chicken monocytes against Salmonella enteritidis, with a minimum impact on inflammatory cytokine production, phagocytosis, and oxidative burst capacities of the cells. Furthermore, feed supplementation with 0.1% butyrate led to a significant increase in HDP gene expression in the intestinal tract of chickens. More importantly, such a feeding strategy resulted in a nearly 10-fold reduction in the bacterial titer in the cecum following experimental infections with S. enteritidis. Collectively, the results indicated that butyrate-induced synthesis of endogenous HDPs is a phylogenetically conserved mechanism of innate host defense shared by mammals and aves, and that dietary supplementation of butyrate has potential for further development as a convenient antibiotic-alternative strategy to enhance host innate immunity and disease resistance.
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影响因子：
5.4
作者：
Bommineni, Yugendar R.;Dai, Huaien;Zhang, Guolong
通讯作者：
Zhang, Guolong
影响因子：
3.1
作者：
LEVISON, ME
通讯作者：
LEVISON, ME
影响因子：
64.5
作者：
BEUG, H;VONKIRCHBACH, A;GRAF, T
通讯作者：
GRAF, T
影响因子：
24.5
作者：
Schauber, J;Svanholm, C;Gudmundsson, GH
通讯作者：
Gudmundsson, GH
DOI：
10.1073/pnas.0707037104
发表时间：
2007-09-18
影响因子：
11.1
作者：
Goitsuka, Ryo;Chen, Chen-Io H.;Cooper, Max D.
通讯作者：
Cooper, Max D.