A versatile and customizable low-cost 3D-printed open standard for microscopic imaging.
A versatile and customizable low-cost 3D-printed open standard for microscopic imaging.
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DOI：
10.1038/s41467-020-19447-9
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发表时间：
2020-11-25
影响因子：
16.6
通讯作者：
Heintzmann R
中科院分区：
文献类型：
--
作者：
Diederich B;Lachmann R;Carlstedt S;Marsikova B;Wang H;Uwurukundo X;Mosig AS;Heintzmann R
用于生物成像的现代显微镜经常以黑匣子的形式出现，其确切的工作原理尚不清楚，其光学分辨率和价格似乎成反比。与UC2(您。看见。也是。)我们提出了一个低成本、3D打印、开源、模块化的显微镜工具箱，并通过实现从概念到实验阶段的完整显微镜开发周期来展示其多功能性。独立的孵化器封闭式Brightfield显微镜在细胞分辨率水平(例如2 μm)连续七天监测单核细胞到巨噬细胞的分化。此外，通过添加非常少的额外组件，几何图形被转移到400欧元的光平板荧光显微镜中，用于对表达绿色荧光蛋白(GFP)的转基因斑马鱼进行体积观察。通过这一点，我们的目标是建立一个开放的光学标准，以促进与各种互补平台的接口。通过使内容和全面的文档公开可用，这里提供的系统有助于轻松、直接地复制、修改和扩展。为了给研究人员提供低成本的解决方案和克服科学中的重复性危机，迫切需要开放标准显微镜。在这里，作者提出了一个3D打印的，开源的模块化显微镜工具箱UC2(你。看见。也是。)只需要几百欧元。
Modern microscopes used for biological imaging often present themselves as black boxes whose precise operating principle remains unknown, and whose optical resolution and price seem to be in inverse proportion to each other. With UC2 (You. See. Too.) we present a low-cost, 3D-printed, open-source, modular microscopy toolbox and demonstrate its versatility by realizing a complete microscope development cycle from concept to experimental phase. The self-contained incubator-enclosed brightfield microscope monitors monocyte to macrophage cell differentiation for seven days at cellular resolution level (e.g. 2 μm). Furthermore, by including very few additional components, the geometry is transferred into a 400 Euro light sheet fluorescence microscope for volumetric observations of a transgenic Zebrafish expressing green fluorescent protein (GFP). With this, we aim to establish an open standard in optics to facilitate interfacing with various complementary platforms. By making the content and comprehensive documentation publicly available, the systems presented here lend themselves to easy and straightforward replications, modifications, and extensions. Open standard microscopy is urgently needed to give low-cost solutions to researchers and to overcome the reproducibility crisis in science. Here the authors present a 3D-printed, open-source modular microscopy toolbox UC2 (You. See. Too.) for a few hundred Euros.
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影响因子：
64.8
作者：
Harris CR;Millman KJ;van der Walt SJ;Gommers R;Virtanen P;Cournapeau D;Wieser E;Taylor J;Berg S;Smith NJ;Kern R;Picus M;Hoyer S;van Kerkwijk MH;Brett M;Haldane A;Del Río JF;Wiebe M;Peterson P;Gérard-Marchant P;Sheppard K;Reddy T;Weckesser W;Abbasi H;Gohlke C;Oliphant TE
通讯作者：
Oliphant TE
影响因子：
3.7
作者：
Diederich B;Wartmann R;Schadwinkel H;Heintzmann R
通讯作者：
Heintzmann R
影响因子：
2.2
作者：
ANDREESEN, R;PICHT, J;LOHR, GW
通讯作者：
LOHR, GW
DOI：
10.1126/science.1257998
发表时间：
2014-10-24
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Chen BC;Legant WR;Wang K;Shao L;Milkie DE;Davidson MW;Janetopoulos C;Wu XS;Hammer JA 3rd;Liu Z;English BP;Mimori-Kiyosue Y;Romero DP;Ritter AT;Lippincott-Schwartz J;Fritz-Laylin L;Mullins RD;Mitchell DM;Bembenek JN;Reymann AC;Böhme R;Grill SW;Wang JT;Seydoux G;Tulu US;Kiehart DP;Betzig E
通讯作者：
Betzig E
影响因子：
3.7
作者：
Cybulski JS;Clements J;Prakash M
通讯作者：
Prakash M