Waves of DNA: Propagating excitations in extended nanoconfined polymers.
Waves of DNA: Propagating excitations in extended nanoconfined polymers.
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DNA 波：在扩展纳米限制聚合物中传播激发。
DOI：
10.1103/physreve.94.042603
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
W. Reisner
中科院分区：
文献类型：
--
作者：
Alexander R. Klotz;H. D. de Haan;W. Reisner
我们使用纳米流体系统来研究沿着单个聚合物链的热驱动集体现象的出现。在我们的方法中，单个DNA分子被限制在蚀刻有嵌入纳米空腔阵列的纳米流体狭缝中；空腔晶格的设计使单链占据多个空腔。荧光视频显微镜数据显示空腔之间的强度波动，包括在跨空腔分子中传播的过量荧光波，对应于空腔中轮廓过密度的传播波动。通过检测邻近空腔之间的强度波动的相关性，可以量化DNA在邻近空腔之间的转移。相关性在衰减之前从反相关最小值增长到相关最大值，对应于在固定的转移时间尺度上相邻腔之间的轮廓转移。观察到的动力学可以用朗格万动力学模拟和耦合扩散的最小晶格模型来建模。这项研究表明，通过将物理系统重新规范化为一系列离散的空腔状态，基于约束的聚合物平衡构型雕刻可以导致新型的动态集体现象。
We use a nanofluidic system to investigate the emergence of thermally driven collective phenomena along a single polymer chain. In our approach, a single DNA molecule is confined in a nanofluidic slit etched with arrays of embedded nanocavities; the cavity lattice is designed so that a single chain occupies multiple cavities. Fluorescent video-microscopy data shows fluctuations in intensity between cavities, including waves of excess fluorescence that propagate across the cavity-straddling molecule, corresponding to propagating fluctuations of contour overdensity in the cavities. The transfer of DNA between neighboring pits is quantified by examining the correlation in intensity fluctuations between neighboring cavities. Correlations grow from an anticorrelated minimum to a correlated maximum before decaying, corresponding to a transfer of contour between neighboring cavities at a fixed transfer time scale. The observed dynamics can be modeled using Langevin dynamics simulations and a minimal lattice model of coupled diffusion. This study shows how confinement-based sculpting of the polymer equilibrium configuration, by renormalizing the physical system into a series of discrete cavity states, can lead to new types of dynamic collective phenomena.
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纳米限制 DNA 的波动模式。
影响因子：
3.2
作者：
Karpusenko,Alena;Carpenter,JoshuaH;Zhou,Chunda;Lim,ShuangFang;Pan,Junhan;Riehn,Robert
通讯作者：
Riehn,Robert
影响因子：
5.5
作者：
Kounovsky-Shafer KL;Hernández-Ortiz JP;Jo K;Odijk T;de Pablo JJ;Schwartz DC
通讯作者：
Schwartz DC
影响因子：
56.9
作者：
Han, J;Craighead, HG
通讯作者：
Craighead, HG
影响因子：
8.6
作者：
Reisner, W;Morton, KJ;Austin, RH
通讯作者：
Austin, RH