DNA sensing by amplifying the number of near-infrared emitting, oligonucleotide-encapsulated silver clusters.
DNA sensing by amplifying the number of near-infrared emitting, oligonucleotide-encapsulated silver clusters.
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DOI：
10.1021/ac201321m
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发表时间：
2011-08-01
影响因子：
7.4
通讯作者：
Degtyareva, Natalya N.
中科院分区：
文献类型：
--
作者：
Petty, Jeffrey T.;Sengupta, Bidisha;Story, Sandra P.;Degtyareva, Natalya N.
双功能寡核苷酸将荧光银簇的原位合成与靶DNA序列的识别整合在一起。与模板C3AC3AC3GC 3A，复合物的形式与10个银原子，具有电子跃迁在近红外线，并检测到在nM浓度使用二极管激光激发。悬垂于该簇编码区，识别组分通过杂交结合靶DNA链，并且复合传感器的这两个区域的解偶联提供用于特定寡核苷酸的模块化传感器。使用猝灭剂链检测靶标，猝灭剂链桥接簇模板和识别组分并干扰簇结合，如静态猝灭所示。竞争性置换的淬灭剂的目标链恢复有利的集群环境，我们的关键发现是，这种交换增强发射通过发射集群的数量成比例增加。通过利用该荧光团的高亮度和近红外光谱区域中固有的低内源性背景，也可以在含血清的缓冲液中完成DNA检测。通过向溶液中补充Ag+，可增强该生物环境中的簇稳定性。
A bifunctional oligonucleotide integrates in situ synthesis of a fluorogenic silver cluster with recognition of a target DNA sequence. With the template C3AC3AC3GC3A, a complex forms with 10 silver atoms that possesses electronic transitions in the near-infrared and that is detected at nM concentrations using diode laser excitation. Pendant to this cluster encoding region, the recognition component binds a target DNA strand through hybridization, and decoupling of these two regions of the composite sensor renders a modular sensor for specific oligonucleotides. A target is detected using a quencher strand that bridges the cluster template and recognition components and disturbs cluster binding, as indicated by static quenching. Competitive displacement of the quencher by the target strand restores the favored cluster environment, and our key finding is that this exchange enhances emission through a proportional increase in the number of emissive clusters. DNA detection is also accomplished in serum-containing buffers, by taking advantage of the high brightness of this fluorophore and the inherently low endogenous background in the near-infrared spectral region. Cluster stability in this biological environment is enhanced by supplementing the solutions with Ag+.
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影响因子：
29.4
作者：
Gwinn, Elisabeth G.;O'Neill, Patrick;Fygenson, Deborah Kuchnir
通讯作者：
Fygenson, Deborah Kuchnir
影响因子：
62.1
作者：
Liu, Juewen;Cao, Zehui;Lu, Yi
通讯作者：
Lu, Yi
影响因子：
4.8
作者：
Nutiu, R;Li, YF
通讯作者：
Li, YF
影响因子：
7.4
作者：
Knemeyer, JP;Marmé, N;Sauer, M
通讯作者：
Sauer, M
DOI：
10.1021/jp908085s
发表时间：
2010-04-01
期刊：
The journal of physical chemistry. B
影响因子：
--
作者：
Loo K;Degtyareva N;Park J;Sengupta B;Reddish M;Rogers CC;Bryant A;Petty JT
通讯作者：
Petty JT