Magnetic transfer contrast accurately localizes substantia nigra confirmed by histology.
Magnetic transfer contrast accurately localizes substantia nigra confirmed by histology.
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DOI：
10.1016/j.biopsych.2012.07.035
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发表时间：
2013-02-01
影响因子：
10.6
通讯作者：
Lahti AC
中科院分区：
文献类型：
--
作者：
Bolding MS;Reid MA;Avsar KB;Roberts RC;Gamlin PD;Gawne TJ;White DM;den Hollander JA;Lahti AC
磁共振成像(MRI)具有多种对比度机制。就像组织学中的各种染色技术一样，每种对比类型揭示了关于大脑结构的不同信息。然而，在MRI中可见的结构如何与先前组织学识别的结构相对应并不总是清楚的。本研究的目的是确定磁转移对比(MTC)和T2对比MRI在显示黑质方面哪个更好。核磁共振扫描是从两个非人类灵长类动物(HAP)体内获得的。随后对NHP进行安乐死、灌流，并将其大脑切片进行组织学分析。每一张切片在切片前都要拍照。每个大脑被分割成大约500，40微米的部分，包括大部分皮质，中脑和后脑的背部部分。选择与解剖MRI图像相对应的水平。用Kluver Barrera(髓鞘和胞体)或酪氨酸羟化酶(TH)(多巴胺能神经元)免疫组织化学染色。使用带有相似性变换的移动最小二乘算法将得到的图像与块脸图像共同配准。MR图像与块面图像类似地被共同配准，使得MRI中的结构可以与组织学图像中的结构相识别。我们发现MTC图像中的高信号(轻)区域与组织学描绘的SN同延。T2加权像上的低信号(暗)区与SN不同范围，但部分延伸至SN，部分延伸至大脑脚。在体内定位SN时，MTC是一种比T2加权更准确的对比机制。
Magnetic Resonance Imaging (MRI) has multiple contrast mechanisms. Like various staining techniques in histology, each contrast type reveals different information about the structure of the brain. However, it is not always clear how structures visible in MRI correspond to structures previously identified by histology. The purpose of this study was to determine if magnetic transfer contrast (MTC) or T2 contrast MRI was better at delineating the substantia nigra. MRI scans were acquired in-vivo from two non-human primates (NHPs). The NHPs were subsequently euthanized, perfused, and their brains sectioned for histological analyses. Each slice was photographed prior to sectioning. Each brain was sectioned into approximately 500, 40-micron sections, encompassing most of the cortex, midbrain, and dorsal parts of the hindbrain. Levels corresponding to anatomical MRI images were selected. From these, adjacent sections were stained using Kluver Barrera (myelin and cell bodies) or tyrosine hydroxylase (TH) (dopaminergic neurons) immunohistochemistry. The resulting images were coregistered to the block-face images using a moving least squares algorithm with similarity transformations. MR images were similarly coregistered to the block-face images, allowing the structures in the MRI to be identified with structures in the histological images. We found that hyperintense (light) areas in MTC images were coextensive with the SN as delineated histologically. The hypointense (dark) areas in T2-weighted images were not coextensive with the SN, but extended partially into the SN and partially into the cerebral peduncles. MTC is a more accurate contrast mechanism than T2-weighting for localizing the SN in vivo.
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影响因子：
10.6
作者：
Shibata, Eri;Sasaki, Makoto;Sakai, Akio
通讯作者：
Sakai, Akio
影响因子：
1.2
作者：
Gawne, Timothy J.
通讯作者：
Gawne, Timothy J.
影响因子：
2.5
作者：
Gawne, Timothy J.
通讯作者：
Gawne, Timothy J.
影响因子：
5
作者：
RUTLEDGE, JN;HILAL, SK;FAHN, S
通讯作者：
FAHN, S
影响因子：
6.2
作者：
Schaefer, Scott;McPhail, Travis;Warren, Joe
通讯作者：
Warren, Joe