Selection of antigenically advanced variants of seasonal influenza viruses.
Selection of antigenically advanced variants of seasonal influenza viruses.
复制标题
DOI：
10.1038/nmicrobiol.2016.58
复制
发表时间：
2016-05-23
影响因子：
28.3
通讯作者：
Kawaoka, Yoshihiro
中科院分区：
文献类型：
--
作者：
Li, Chengjun;Hatta, Masato;Burke, David F.;Ping, Jihui;Zhang, Ying;Ozawa, Makoto;Taft, Andrew S.;Das, Subash C.;Hanson, Anthony P.;Song, Jiasheng;Imai, Masaki;Wilker, Peter R.;Watanabe, Tokiko;Watanabe, Shinji;Ito, Mutsumi;Iwatsuki-Horimoto, Kiyoko;Russell, Colin A.;James, Sarah L.;Skepner, Eugene;Maher, Eileen A.;Neumann, Gabriele;Klimov, Alexander I.;Kelso, Anne;McCauley, John;Wang, Dayan;Shu, Yuelong;Odagiri, Takato;Tashiro, Masato;Xu, Xiyan;Wentworth, David E.;Katz, Jacqueline M.;Cox, Nancy J.;Smith, Derek J.;Kawaoka, Yoshihiro
流感病毒变异频繁，需要不断更新疫苗病毒。为了建立可以补充当前疫苗株选择过程的实验方法，我们通过将人和/或雪貂恢复期血清与人H1N1和H3 N2病毒一起孵育，从人H1N1和H3 N2流感病毒文库中选择了在血凝素蛋白的球状头部（包括抗原位点）具有随机突变的抗原变体。我们还从用恢复期血清处理的人病毒和先前已对人流感病毒免疫的小鼠中选择了抗原逃逸变体。我们对过去的流感病毒进行的试点研究确定了逃逸突变体，这些突变体在抗原性上与自然界中出现的变体相似，从而确立了我们方法的可行性。我们对当代人类流感病毒的研究在2014-2015年引起流行病之前发现了逃逸突变体。这种方法可能有助于预测潜在的抗原逃逸变体，并在它们在自然界中广泛传播之前选择未来的候选疫苗。本文的在线版本（doi：10.1038/nmicrobiol.2016.58）包含补充材料，可供授权用户使用。从人H1N1和H3 N2流感病毒库中的血凝素蛋白中具有随机突变的抗原变体，通过与人和/或雪貂恢复期血清孵育选择，鉴定出与自然界中出现的那些相似的逃逸变体。本文的在线版本（doi：10.1038/nmicrobiol.2016.58）包含补充材料，可供授权用户使用。
Influenza viruses mutate frequently, necessitating constant updates of vaccine viruses. To establish experimental approaches that may complement the current vaccine strain selection process, we selected antigenic variants from human H1N1 and H3N2 influenza virus libraries possessing random mutations in the globular head of the haemagglutinin protein (which includes the antigenic sites) by incubating them with human and/or ferret convalescent sera to human H1N1 and H3N2 viruses. We also selected antigenic escape variants from human viruses treated with convalescent sera and from mice that had been previously immunized against human influenza viruses. Our pilot studies with past influenza viruses identified escape mutants that were antigenically similar to variants that emerged in nature, establishing the feasibility of our approach. Our studies with contemporary human influenza viruses identified escape mutants before they caused an epidemic in 2014–2015. This approach may aid in the prediction of potential antigenic escape variants and the selection of future vaccine candidates before they become widespread in nature. The online version of this article (doi:10.1038/nmicrobiol.2016.58) contains supplementary material, which is available to authorized users. Antigenic variants from human H1N1 and H3N2 influenza virus libraries possessing random mutations in the haemagglutinin protein, selected by incubation with human and/or ferret convalescent sera, identify escape variants similar to those that have emerged in nature. The online version of this article (doi:10.1038/nmicrobiol.2016.58) contains supplementary material, which is available to authorized users.
登录
查看更多内容
影响因子：
3.7
作者：
Martín, J;Wharton, SA;Steinhauer, DA
通讯作者：
Steinhauer, DA
影响因子：
6.4
作者：
O'Donnell CD;Vogel L;Wright A;Das SR;Wrammert J;Li GM;McCausland M;Zheng NY;Yewdell JW;Ahmed R;Wilson PC;Subbarao K
通讯作者：
Subbarao K
影响因子：
8.8
作者：
Chambers BS;Parkhouse K;Ross TM;Alby K;Hensley SE
通讯作者：
Hensley SE
影响因子：
64.5
作者：
CATON, AJ;BROWNLEE, GG;GERHARD, W
通讯作者：
GERHARD, W
影响因子：
5.8
作者：
Neher, Richard A.;Bedford, Trevor
通讯作者：
Bedford, Trevor