The airway inflammation induced by nasal inoculation of PM2.5 and the treatment of bacterial lysates in rats.
The airway inflammation induced by nasal inoculation of PM2.5 and the treatment of bacterial lysates in rats.
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鼻腔接种PM2.5致大鼠气道炎症及细菌裂解物处理
DOI：
10.1038/s41598-018-28156-9
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发表时间：
2018-06-29
影响因子：
4.6
通讯作者：
Hong SL
中科院分区：
文献类型：
--
作者：
Shen Y;Zhang ZH;Hu D;Ke X;Gu Z;Zou QY;Hu GH;Song SH;Kang HY;Hong SL
颗粒物（PM）是我国最重要的环境问题之一。本研究旨在探讨PM2.5与健康大鼠气道炎症的相关性。PM2.5组连续15天鼻内滴入PM2.5混悬液，第16 ~ 90天口服生理盐水。BV干预组与PM2.5暴露组相同，但每日给予BV而非生理盐水。进行组织病理学检查以评估气道炎症。采用流式细胞术和ELISA法检测Th 1/Th 2/Treg/Th 17细胞的比例和功能。Western blot和real-time PCR检测AhR的表达。结果发现，PM2. 5暴露后气道上皮细胞损伤，炎性细胞浸润增加; PM2. 5组Th细胞免疫失衡; PM2. 5暴露后气道AhR表达增加。在PM2.5 + BV组中，我们证明了气道中免疫失衡的减轻和炎性细胞浸润的减少。我们的研究表明，暴露于PM2.5诱导气道炎症。Th 1/Th 2/Treg/Th 17失衡可能与AhR通路的激活有关。口服BV可降低PM2.5诱导的大鼠气道炎症并调节全身免疫反应。
Particulate matter (PM) is one of the most important environmental issues in China. This study aimed to explore the correlation between PM2.5 and airway inflammation in healthy rats. The PM2.5 group was given an intranasal instillation of PM2.5 suspension on 15 consecutive days, and each received oral saline from day 16 to 90. The BV intervention group was treated as the PM2.5 exposure group, except that BV instead of saline was given daily. A histopathologic examination was performed to evaluate the airway inflammation. The prevalence and function of Th1/Th2/Treg/Th17 cells were detected by flow cytometry and ELISA. The expression of AhR was detected by western blot and real-time PCR. We found that epithelial damage and increased infiltration of inflammatory cell were present in the airways after PM2.5 exposure; there was an immune imbalance of Th cells in the PM2.5 group; the expression of AhR was increased in the airways after PM2.5 exposure. In the PM2.5 + BV group, we demonstrated alleviated immune imbalance and reduced inflammatory cell infiltration in the airways. Our study showed that exposure to PM2.5 induced airway inflammation. The imbalance of Th1/Th2/Treg/Th17 in PM2.5-induced airway inflammation might be associated with activation of the AhR pathway. Oral BV reduces PM2.5-induced airway inflammation and regulates systemic immune responses in rats.
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DOI：
10.1016/j.ijporl.2013.01.009
发表时间：
2013-05-01
影响因子：
1.5
作者：
Bitar, Mohamed A.;Saade, Rami
通讯作者：
Saade, Rami
影响因子：
3.7
作者：
Ogino K;Zhang R;Takahashi H;Takemoto K;Kubo M;Murakami I;Wang DH;Fujikura Y
通讯作者：
Fujikura Y
影响因子：
2.1
作者：
Cassee FR;Héroux ME;Gerlofs-Nijland ME;Kelly FJ
通讯作者：
Kelly FJ
影响因子：
14.2
作者：
Molet, S;Hamid, Q;Chakir, J
通讯作者：
Chakir, J
影响因子：
5
作者：
Koatz, Ana M.;Coe, Noemi A.;Alter, Adriana J.
通讯作者：
Alter, Adriana J.