Allergic airway inflammation by nasal inoculation of particulate matter (PM2.5) in NC/Nga mice.
Allergic airway inflammation by nasal inoculation of particulate matter (PM2.5) in NC/Nga mice.
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DOI：
10.1371/journal.pone.0092710
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Fujikura Y
中科院分区：
文献类型：
--
作者：
Ogino K;Zhang R;Takahashi H;Takemoto K;Kubo M;Murakami I;Wang DH;Fujikura Y
为评价冬季空气悬浮颗粒物2.5 （PM2.5）对气道炎症的影响，对螨过敏原高敏感的NC/Nga小鼠接种PM2.5水溶性上清液（Sup）和水不溶性沉淀物（Pre）。腹腔注射含氧化铝的汤致敏。5 d后，在NC/Nga小鼠中经鼻路接种Sup、Pre或Sup和Pre均接种5次以提高致敏性，并在第11天进行攻毒接种。第12天，检测小鼠气道高反应性（AHR）、BALF细胞计数和IL-1β浓度、Th1和Th2细胞因子mRNA表达、趋化因子eotaxin 1和eotaxin 2、炎性体复合物分子IL-1β、caspase 1、核苷酸结合域和富亮氨酸重复蛋白3 （NLRP3）在肺组织中的表达及组织病理学变化。Sup和Pre的协同作用表现在AHR、BALF细胞、IL-13、eotaxin1和IL-1β mRNA表达和BALF中IL-1β浓度的增加。Sup和Pre组小鼠炎性肉芽周围巨噬细胞内可见不溶性颗粒沉积。这些结果表明，PM2.5可通过炎症小体相关机制以及不溶性颗粒和可溶性成分的协同作用诱导螨虫过敏原遗传高敏感小鼠气道高反应性。
To evaluate the effect of airborne particulate matter 2.5 (PM2.5) in winter on airway inflammation, water-soluble supernatant (Sup) and water-insoluble precipitate (Pre) in PM2.5 were inoculated in NC/Nga mice with high sensitivity to mite allergens. Sup with aluminum oxide was injected intraperitoneally for sensitization. Five days later, Sup, Pre or both Sup and Pre were inoculated via the nasal route five times for more sensitization and a challenge inoculation on the 11th day in NC/Nga mice. On the 12th day, mice were examined for airway hyperresponsiveness (AHR), BALF cell count and IL-1β concentration, mRNA expression of Th1 and Th2 cytokines, chemokines such as eotaxin 1 and eotaxin 2, inflammasomal complex molecules such as IL-1β, caspase 1 and the nucleotide-binding domain and leucine-rich repeat protein 3 (NLRP3) in lung tissue as well as histopathology. The synergistic effect of Sup and Pre was observed in terms of increases in AHR, BALF cells, the mRNA expression of IL-13, eotaxin1 and IL-1β, and the IL-1β concentration in BALF. Intracellular deposits of insoluble particulates were observed in macrophages around inflammatory granulation of the mouse group treated with Sup and Pre. These results suggest that PM2.5 can induce airway hyperresponsiveness in mice with genetically high sensitivity to mite allergens by an inflammasome-associated mechanism and synergistic action of insoluble particulates and soluble components.
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影响因子：
56.9
作者：
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影响因子：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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