Prolactin inhibits plasminogen activator activity in the preovulatory follicles.
Prolactin inhibits plasminogen activator activity in the preovulatory follicles.
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催乳素抑制排卵前卵泡中纤溶酶原激活剂的活性。
DOI：
10.1210/endo-126-1-631
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发表时间：
1990
期刊：
影响因子：
4.8
通讯作者：
Y. Nakamura
中科院分区：
文献类型：
--
作者：
Y. Yoshimura;K. Maruyama;M. Shiraki;S. Kawakami;M. Fukushima;Y. Nakamura
本研究旨在确定PRL对排卵前卵泡形态和纤溶酶原激活物（PA）活性变化的影响。分别以10、10(2)、10(3)ng/ml分别灌注hCG或hCG +兔卵巢。10(3) ng/ml的PRL直接抑制hCG暴露诱导的表面上皮细胞的退化和分解。表面下结缔组织通过十二烷基硫酸钠处理可见，去除卵巢表面上皮细胞，从而暴露胶原原纤维和基底层。十二烷基硫酸钠处理显示在prl处理的卵巢卵泡壁顶端的结缔组织破坏的抑制。灌注hCG后，兔卵巢成熟卵泡PA活性从1.40 +/- 0.08增加到28.4 +/- 4.25 IU/g。在灌注液中加入PRL以剂量依赖性的方式抑制了hcg刺激的滤泡内PA活性增加。虽然在7小时时，单独用hCG处理的成熟卵泡比用hCG加PRL处理的卵泡内PA活性更高，但这种差异并不显著。这些结果表明，PRL可能通过抑制卵泡内组织PA活性，直接通过干扰卵巢内成熟卵泡破裂所需的机械事件起作用。
The present study was designed to determine the effects of PRL on changes in morphology and plasminogen activator (PA) activity in the preovulatory follicles. Rabbit ovaries were perfused with hCG alone or with hCG plus at 10, 10(2), or 10(3) ng/ml. PRL at 10(3) ng/ml directly inhibited the degeneration and decomposition of surface epithelial cells induced by hCG exposure. The subsurface connective tissue was visualized by treatment with sodium dodecyl sulfate, which removed surface epithelial cells from the ovary, thereby exposing collagen fibrils and the basal lamina. Sodium dodecyl sulfate treatment revealed inhibition of connective tissue disruption at the apex of the follicle wall in PRL-treated ovaries. PA activity in mature follicles in perfused rabbit ovaries exposed to hCG increased from 1.40 +/- 0.08 to 28.4 +/- 4.25 IU/g tissue after 4 h of perfusion. The addition of PRL to the perfusate inhibited the hCG-stimulated increase in intrafollicular PA activity in a dose-dependent fashion. Although at 7 h mature follicles treated by hCG alone showed greater intrafollicular PA activity than those treated with hCG plus PRL, this difference was not significant. These results suggest that PRL may act directly by interfering with mechanical events within the ovary that are required for the rupture of mature Graafian follicles, probably via the inhibition of intrafollicular tissue PA activity.
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大鼠卵巢中的纤溶酶原激活剂。
DOI：
--
发表时间：
1985
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Canipari,R;Strickland,S
通讯作者：
Strickland,S
影响因子：
9.8
作者：
Yoshimura,Y;Santulli,R;Atlas,SJ;Fujii,S;Wallach,EE
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Wallach,EE
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影响因子：
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作者：
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LeMaire,WJ
假妊娠期间离体灌注兔卵巢黄体分泌的黄体酮。
影响因子：
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作者：
Dharmarajan,AM;Yoshimura,Y;Sueoka,K;Atlas,SJ;Dubin,NH;Ewing,LL;Zirkin,BR;Wallach,EE
通讯作者：
Wallach,EE
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影响因子：
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作者：
Kobayashi,Y;Wright,KH;Santulli,R;Wallach,EE
通讯作者：
Wallach,EE