NisI maturation and its influence on nisin resistance in Lactococcus lactis
NisI maturation and its influence on nisin resistance in Lactococcus lactis
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乳酸乳球菌NisI成熟及其对乳酸链球菌素耐药性的影响
DOI：
10.1128/aem.01306-20
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发表时间：
2020-07
影响因子：
4.4
通讯作者：
Qiao Jianjun
中科院分区：
文献类型：
--
作者：
Liu Jiaheng;Xiong Hui;Du Yuhui;Wiwatanaratanabutr Itsanun;Wu Xiaofang;Zhao Guangrong;Zhu Hongji;Caiyin Qinggele;Qiao Jianjun
NisI具有对nisin的免疫力，具有高底物特异性，以防止产生nisin的乳酸乳球菌菌株的自杀效应。然而，NisI的成熟过程及其对nisin耐药的影响尚未被描述。本文报道了脂蛋白信号肽酶II （Lsp）和脂蛋白二酰基甘油转移酶（Lgt）在乳酸菌F44 NisI成熟和耐乳素过程中的作用。我们发现，一株lsp缺陷突变体对nisin的抗性显著降低，而生长没有显著差异。我们在体外证明了Lsp可以切割NisI前体的信号肽。此外，Lgt催化的二酰基甘油修饰对NisI在细胞包膜上的附着起决定性作用，而对NisI前体信号肽的切割没有影响。通过表面等离子体共振（SPR）分析，nisin与NisI相互作用的解离常数（KD）比nisin与NisI前体与NisI相互作用的解离常数（KD）增加了2.8倍，证明了lp催化的信号肽裂解对NisI的免疫活性至关重要。我们的研究揭示了乳酸菌中NisI的成熟过程，并提出了提高工业NisI生产的潜在策略。
NisI confers immunity against nisin, with high substrate specificity to prevent a suicidal effect in nisin-producing Lactococcus lactis strains. However, the NisI maturation process as well as its influence on nisin resistance has not been characterized. Here, we report the roles of lipoprotein signal peptidase II (Lsp) and prolipoprotein diacylglyceryl transferase (Lgt) in NisI maturation and nisin resistance of L. lactis F44. We found that the resistance of nisin of an Lsp-deficient mutant remarkably decreased, while no significant differences in growth were observed. We demonstrated that Lsp could cleave signal peptide of NisI precursor in vitro. Moreover, diacylglyceryl modification of NisI catalyzed by Lgt played a decisive role in attachment of NisI on the cell envelope, while it exhibited no effects on cleavage of the signal peptides of NisI precursor. The dissociation constant (KD) for the interaction between nisin and NisI exhibited a 2.8-fold increase compared with that between nisin and pre-NisI with signal peptide by surface plasmon resonance (SPR) analysis, providing evidence that Lsp-catalyzed signal peptide cleavage was critical for the immune activity of NisI. Our study revealed the process of NisI maturation in L. lactis and presented a potential strategy to enhance industrial nisin production.
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DOI：
10.1074/jbc.274.3.1698
发表时间：
1999-01
期刊：
The Journal of Biological Chemistry
影响因子：
--
作者：
H. Tjalsma;V. Kontinen;Z. Prágai;Ho-Chen Wu;Rob Meima;G. Venemâ;S. Bron;M. Sarvas;J. V. van Dijl-J.-V.
通讯作者：
H. Tjalsma;V. Kontinen;Z. Prágai;Ho-Chen Wu;Rob Meima;G. Venemâ;S. Bron;M. Sarvas;J. V. van Dijl-J.-V.
影响因子：
2.1
作者：
O. Koponen;T. Takala;U. Saarela;M. Qiao;P. Saris
通讯作者：
O. Koponen;T. Takala;U. Saarela;M. Qiao;P. Saris
影响因子：
3.4
作者：
Ö. Şimşek;A. Çon;N. Akkoç;P. Saris;M. Akçelik
通讯作者：
Ö. Şimşek;A. Çon;N. Akkoç;P. Saris;M. Akçelik
影响因子：
4.1
作者：
Alkhatib, Zainab;Abts, Andre;Smits, Sander H. J.
通讯作者：
Smits, Sander H. J.
影响因子：
15
作者：
GROSS, E;MORELL, JL
通讯作者：
MORELL, JL