Expression of CD34 and α-SMA Markers in Oral Squamous Cell Carcinoma Differentiation. A Histological and Histo-Chemical Study.
Expression of CD34 and α-SMA Markers in Oral Squamous Cell Carcinoma Differentiation. A Histological and Histo-Chemical Study.
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DOI：
10.3390/ijerph18010192
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发表时间：
2020-12-29
影响因子：
--
通讯作者：
Abduljabbar T
中科院分区：
文献类型：
--
作者：
Maqsood A;Ali A;Zaffar Z;Mokeem S;Mokeem SS;Ahmed N;Al-Hamoudi N;Vohra F;Javed F;Abduljabbar T
为了降低OSCC病例的发病率和死亡率，早期诊断、行为评估和预后评估至关重要。本研究分析CD34和α-平滑肌肌动蛋白（α-SMA）在OSCC中的表达，探讨其在OSCC诊断和预后中的意义。在多个癌症治疗中心诊断为OSCC的原发病例包括切除活检。组织切片包埋，H & E染色，观察组织分化及肿瘤血管浸润情况。采用CD34抗体和α-SMA抗体进行免疫组化。数据分析采用卡方检验和Pearson相关系数(r)检验。80例患者，男性50例（62.5%），女性30例（37.5%），平均年龄45±14.1岁。口腔黏膜是OSCC最常见的病变部位[36 (45%)]；中分化占47.5%，高分化占33.8%。35%的标本可见肿瘤血管浸润。CD34的表达与OSCC的组织学分级有显著相关性（p < 0.002）。在所有低分化OSCC标本中，cd34表达低，α-SMA表达高。cd34是OSCC诊断的关键预后因素，α- sma阳性肌成纤维细胞增加可能提示侵袭性OSCC行为。
To reduce morbidity and mortality rates of OSCC cases, early diagnosis, assessment of behavior and prognostic estimates are vital. This study analyzed the expression of CD34 and alpha smooth muscle actin (α-SMA) in OSCC, to establish their significance in diagnosis and prognosis. Primary cases of OSCC, diagnosed with excisional biopsy at multiple cancer treatment centers, were included. Tissue sections were embedded and stained with H & E for histological differentiation and invasion of tumor vessel. Immunohistochemistry was performed using antibodies against CD34 and α-SMA. The chi-square and Pearson correlation coefficient (r) tests were applied for data analysis. Eighty patients with fifty males (62.5%) and thirty females (37.5%) and mean age of 45 ± 14.1 years were evaluated. Buccal mucosa was the most common site for OSCC lesions [36 (45%)]; 47.5% of lesions were moderately differentiated and 33.8% were well-differentiated lesions. Invasion of tumor vessels was observed in 35% of specimens. A significant association was seen between CD34 expression and histological grading of OSCC (p < 0.002). Among all poorly differentiated OSCC specimens, expression of CD 34 was low and α-SMA was high. CD 34 is a critical prognostic factor in OSCC diagnosis and increased α-SMA-positive myofibroblasts may indicate aggressive OSCC behavior.
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影响因子：
5.9
作者：
Byakodi, Raghavendra;Byakodi, Sanjay;Mahind, Prashant
通讯作者：
Mahind, Prashant
影响因子：
4.8
作者：
Bello, I. O.;Alanen, K.;Salo, T.
通讯作者：
Salo, T.
影响因子：
3.3
作者：
Kojc, N;Zidar, N;Gale, N
通讯作者：
Gale, N
DOI：
10.31557/apjcp.2020.21.4.1115
发表时间：
2020-04-01
期刊：
Asian Pacific journal of cancer prevention : APJCP
影响因子：
--
作者：
Raffat, Muhammad Arsalan;Hadi, Naila Irum;Vohra, Fahim
通讯作者：
Vohra, Fahim
影响因子：
4.8
作者：
Guttman, D;Stern, Y;Feinmesser, R
通讯作者：
Feinmesser, R