Decreased expression of nucleoside diphosphate kinase α isoform, an nm23‐H2 gene homolog, is associated with metastatic potential of rat mammary‐adenocarcinoma cells
Decreased expression of nucleoside diphosphate kinase α isoform, an nm23‐H2 gene homolog, is associated with metastatic potential of rat mammary‐adenocarcinoma cells
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核苷二磷酸激酶 α 亚型（nm23-H2 基因同源物）的表达减少与大鼠乳腺癌细胞的转移潜力相关
DOI：
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发表时间：
1996
影响因子：
6.4
通讯作者：
N. Kimura
中科院分区：
文献类型：
--
作者：
M. Fukuda;A. Ishii;Y. Yasutomo;N. Shimada;N. Ishikawa;N. Hanai;N. Nagata;T. Irimura;G. Nicolson;N. Kimura
nm 23基因[编码核苷二磷酸激酶（NDPK）]可能在某些肿瘤细胞中起转移抑制剂的作用。我们分别使用低（MTC）和高（MTLn 3）自发转移潜能的大鼠乳腺癌细胞系研究了NDPK亚型（α和β）在转移过程中的作用。在这些细胞系中，与大多数大鼠组织一样，α亚型（nm 23-H2同系物）在mRNA和蛋白质水平上的表达高于β亚型（nm 23-H1同系物）。当通过北方印迹和Western印迹分析检查时，与转移性差的MTC细胞相比，在高转移性MTLn 3细胞中2种亚型的表达降低。表达减少也与细胞提取物中NDPK酶活性降低相关。Southern印迹和RT-PCR-SSCP分析表明，这2个基因在其翻译区没有发生明显改变或突变。转染NDPKα和NDPKβ cDNA的MTLn 3细胞克隆植入同系大鼠乳腺脂肪垫后均具有致瘤性。其中，只有转染NDPKα基因的克隆在自发转移试验中表现出肺转移减少。© 1996 Wiley利斯公司
The nm23 gene [encoding nucleoside diphosphate kinase (NDPK)] may act as a metastasis suppressor in certain tumor cells. We investigated the role of NDPK isoforms (α and β) in the metastatic processes, using rat mammary‐adenocarcinoma cell lines of poor (MTC) and high (MTLn3) spontaneous metastatic potential respectively. In these cell lines, as in most rat tissues, the α isoform (nm23‐H2 homolog) was more highly expressed than the β isoform (nm23‐H1 homolog) at the mRNA and protein levels. When examined by Northern‐ and Western‐blot analyses, expression of the 2 isoforms was reduced in highly metastatic MTLn3 cells compared with poorly metastatic MTC cells. The reduced expression was also associated with diminished NDPK‐enzyme activity in the cell extracts. Southern‐blot and RT‐PCR‐SSCP analyses suggested that the 2 genes were not grossly altered or mutated in their translation regions. MTLn3 cell clones transfected with NDPKα or NDPKβ cDNA were all tumorigenic when implanted into the mammary fat pad of syngeneic rats. Among those, only clones transfected with the NDPKα gene exhibited reduced lung metastasis in a spontaneous metastasis assay. © 1996 Wiley‐Liss, Inc.
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影响因子：
8
作者：
Z. Su;V. Austin;S. Zimmer;P. Fisher
通讯作者：
Z. Su;V. Austin;S. Zimmer;P. Fisher
DOI：
10.1093/jnci/68.3.507
发表时间：
1982-03
期刊：
Journal of the National Cancer Institute
影响因子：
--
作者：
A. Neri;D. Welch;T. Kawaguchi;G. Nicolson
通讯作者：
A. Neri;D. Welch;T. Kawaguchi;G. Nicolson
DOI：
10.1016/s0021-9258(17)37011-4
发表时间：
1994-03
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
E. Postel;C. Ferrone
通讯作者：
E. Postel;C. Ferrone
影响因子：
11.2
作者：
J. Kantor;Beth A. McCormick;Patricia S. Steeg;Bruce R. Zetter
通讯作者：
J. Kantor;Beth A. McCormick;Patricia S. Steeg;Bruce R. Zetter
影响因子：
10.3
作者：
STEEG, PS;BEVILACQUA, G;SOBEL, ME
通讯作者：
SOBEL, ME