Characterization of SARS-CoV-2-Specific Humoral and Cellular Immune Responses Induced by Inactivated COVID-19 Vaccines in a Real-World Setting.
Characterization of SARS-CoV-2-Specific Humoral and Cellular Immune Responses Induced by Inactivated COVID-19 Vaccines in a Real-World Setting.
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DOI：
10.3389/fimmu.2021.802858
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发表时间：
2021
影响因子：
7.3
通讯作者：
Liu J
中科院分区：
文献类型：
--
作者：
Li Z;Xiang T;Liang B;Deng H;Wang H;Feng X;Quan X;Wang X;Li S;Lu S;Yang X;Wang B;Zelinskyy G;Trilling M;Sutter K;Lu M;Dittmer U;Yang D;Zheng X;Liu J
虽然针对2019冠状病毒病（COVID-19）的灭活疫苗的免疫原性已在多项进行良好的临床试验中得到表征，但目前缺乏关于此类疫苗引起的针对严重急性呼吸道综合征冠状病毒2（SARS-CoV-2）的免疫应答的真实证据。在这里，我们全面表征了在真实条件下由灭活的COVID-19疫苗在126名个体中诱导的SARS-CoV-2特异性细胞和体液免疫应答的各种参数。两针免疫后，87.06%（74/85）的个体检出S受体结合域IgG（S-RBD IgG），78.82%（67/85）的个体检出中和抗体（NAb）。与男性接种者相比，女性参与者的S-RBD IgG和NAb浓度更高。有趣的是，第一次和第二次疫苗接种之间较长的给药间隔导致较好的长期SARS-CoV-2 S-RBD IgG应答。在接受两剂疫苗的个体中，产生效应细胞因子（IFN-γ、IL-2和TNF-α）的CD 4 + T细胞的频率显著高于接受一剂疫苗的个体和未接种疫苗的个体，这些效应细胞因子响应于对应于SARS-CoV-2刺突（S）、核衣壳（N）或膜（M）蛋白的肽池的刺激。S、N或M特异性CD 4+和CD 8 + T细胞应答分别在95.83%（69/72）和54.16%（39/72）的双重疫苗接种个体中检出。纵向分析表明，识别S、N和M的CD 4 + T细胞应答在单次接种疫苗后迅速减弱，但在第二次接种后得到加强并变得更加持久。总体而言，我们提供了在现实世界环境中由灭活COVID-19疫苗诱导的免疫应答的全面表征，表明在接种两剂灭活COVID-19疫苗后，大多数个体都引发了体液和细胞SARS-CoV-2特异性免疫。
While the immunogenicity of inactivated vaccines against coronavirus disease 2019 (COVID‐19) has been characterized in several well-conducted clinical trials, real-world evidence concerning immune responses against severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) raised by such vaccines is currently missing. Here, we comprehensively characterized various parameters of SARS-CoV-2-specific cellular and humoral immune responses induced by inactivated COVID-19 vaccines in 126 individuals under real-world conditions. After two doses of vaccination, S-receptor binding domain IgG (S-RBD IgG) and neutralizing antibody (NAb) were detected in 87.06% (74/85) and 78.82% (67/85) of individuals, respectively. Female participants developed higher concentrations of S-RBD IgG and NAb compared to male vaccinees. Interestingly, a longer dosing interval between the first and second vaccination resulted in a better long-term SARS-CoV-2 S-RBD IgG response. The frequencies of CD4+ T cells that produce effector cytokines (IFN-γ, IL-2, and TNF-α) in response to stimulation with peptide pools corresponding to the SARS-CoV-2 spike (S), nucleocapsid (N) or membrane (M) protein were significantly higher in individuals received two doses of vaccine than those received one dose of vaccine and unvaccinated individuals. S, N, or M-specific CD4+ and CD8+ T cell responses were detectable in 95.83% (69/72) and 54.16% (39/72) of double-vaccinated individuals, respectively. The longitudinal analysis demonstrated that CD4+ T cell responses recognizing S, N, and M waned quickly after a single vaccine dose, but were boosted and became more sustained following a second dose. Overall, we provide a comprehensive characterization of immune responses induced by inactivated COVID-19 vaccines in real-world settings, suggesting that both humoral and cellular SARS-CoV-2-specific immunity are elicited in the majority of individuals after two doses of inactivated COVID-19 vaccines.
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影响因子：
7.3
作者：
Wang Q;Pan W;Liu Y;Luo J;Zhu D;Lu Y;Feng X;Yang X;Dittmer U;Lu M;Yang D;Liu J
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影响因子：
64.5
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PITCH Consortium
DOI：
10.1038/nri2394
发表时间：
2008-09
期刊：
Nature reviews. Immunology
影响因子：
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作者：
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通讯作者：
Fish EN
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DOI：
10.1016/s1473-3099(20)30831-8
发表时间：
2021-01
期刊：
The Lancet. Infectious diseases
影响因子：
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作者：
Xia S;Zhang Y;Wang Y;Wang H;Yang Y;Gao GF;Tan W;Wu G;Xu M;Lou Z;Huang W;Xu W;Huang B;Wang H;Wang W;Zhang W;Li N;Xie Z;Ding L;You W;Zhao Y;Yang X;Liu Y;Wang Q;Huang L;Yang Y;Xu G;Luo B;Wang W;Liu P;Guo W;Yang X
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Yang X
DOI：
10.1016/s1473-3099(10)70049-9
发表时间：
2010-05
期刊：
The Lancet. Infectious diseases
影响因子：
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作者：
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通讯作者：
Pekosz A