CCR7 plays no appreciable role in trafficking of central memory CD4 T cells to lymph nodes.
CCR7 plays no appreciable role in trafficking of central memory CD4 T cells to lymph nodes.
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DOI：
10.4049/jimmunol.1200938
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发表时间：
2013-09-15
期刊：
影响因子：
--
通讯作者：
Campbell JJ
中科院分区：
文献类型：
--
作者：
Vander Lugt B;Tubo NJ;Nizza ST;Boes M;Malissen B;Fuhlbrigge RC;Kupper TS;Campbell JJ
CCR 7 −/−小鼠在LN和脾脏结构方面表现出严重的异常，这使得CCR 7介导的T细胞体内运输的研究变得复杂。为了解决这个问题，我们建立了体内模型，其中WT和CCR 7 −/−群体在具有正常淋巴器官的小鼠中共存，并且必须在这些小鼠的组织中竞争发育小生境。在我们在体内创造的条件下，我们发现记忆性CD 4 T细胞从血液进入LN不依赖于CCR 7。因此，通过LN运输的中央记忆性CD 4 T细胞（通常由其CCR 7的表达来定义）似乎没有通过表达该受体获得任何竞争性归巢优势。此外，与皮肤DC群体相反，我们发现CCR 7缺陷对CD 4 T细胞从发炎皮肤中的退出没有明显影响。最后，我们发现WT和CCR 7 −/−前体在主要的胸腺亚群中同样存在，尽管之前的研究发现CCR 7在从骨髓来源的T细胞前体接种胸腺中起作用。
CCR7−/− mice exhibit profound anomalies in LN and spleen architecture, which complicates the study of CCR7-mediated T cell trafficking in vivo. To circumvent this problem, we established in vivo models in which WT and CCR7−/− populations coexist within mice possessing normal lymphoid organs, and must compete for developmental niches within the tissues of these mice. Under the conditions we have created in vivo, we find the entry of memory CD4 T cells into LN from the blood to be independent of CCR7. Thus, the central memory CD4 T cells that traffic though LN, which are often defined by their expression of CCR7, do not appear to gain any competitive homing advantage by expressing this receptor. Furthermore, in contrast to cutaneous DC populations, we found that CCR7 deficiency had no appreciable effect on the exit of CD4 T cells from inflamed skin. Finally, we found that WT and CCR7−/− precursors were equally represented within the major thymic subpopulations, despite previous findings that CCR7 plays a role in seeding the thymus from bone marrow-derived T cell precursors.
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影响因子：
56.9
作者：
Campbell, JJ;Hedrick, J;Butcher, EC
通讯作者：
Butcher, EC
DOI：
10.1084/jem.20031645
发表时间：
2004-04-19
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Scimone ML;Felbinger TW;Mazo IB;Stein JV;Von Andrian UH;Weninger W
通讯作者：
Weninger W
DOI：
10.1084/jem.171.3.801
发表时间：
1990-03-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Mackay CR;Marston WL;Dudler L
通讯作者：
Dudler L
影响因子：
6.4
作者：
Bjorkdahl, O;Barber, KA;Thomsen, LL
通讯作者：
Thomsen, LL
影响因子：
4.4
作者：
Campbell, JJ;Murphy, KE;Wu, LJ
通讯作者：
Wu, LJ