An aptamer biosensor for CA125 quantification in human serum based on upconversion luminescence resonance energy transfer
An aptamer biosensor for CA125 quantification in human serum based on upconversion luminescence resonance energy transfer
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基于上转换发光共振能量转移的人血清 CA125 定量适体生物传感器
DOI：
10.1016/j.microc.2020.105761
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发表时间：
2021-02
影响因子：
4.8
通讯作者：
Chunya Li
中科院分区：
文献类型：
--
作者：
Xiaojing Zhang;Yanying Wang;Hongping Deng;Xiaoxing Xiong;Huijuan Zhang;Tao Liang;Chunya Li
CA125是卵巢癌早期检测中应用最广泛的生物标志物。基于发光共振能量转移（LRET）的探针检测CA125具有明显的特异性和简单性，但它们受到生物样品自身荧光的强烈干扰。为了解决这一问题，采用近红外可激发上转换纳米颗粒（UCNPs）作为能量供体制备CA125生物传感器。利用碳点（CDs）作为能量受体，实现了UCNPs的上转换发光（UCL）光谱与CDs的吸收光谱的重叠。aptamer修饰的UCNPs通过π-π堆叠相互作用与CDs结合，触发LRET过程，诱导UCL猝灭，猝灭效率高达90%。CA125存在时，CA125-适体复合物的形成阻断了π-π堆积，恢复了UCL。在0.01 ~ 100 U·mL−1范围内，随CA125浓度的对数增加，UCL强度呈线性增加。检出限为9.0 × 10−3U·mL−1。该探针也可用于人血清CA125的检测，可能是卵巢癌早期检测的有用工具。
CA125 is the most widely used biomarker for the early detection of ovarian cancer. Luminescence resonance energy transfer (LRET)-based probes show pronounced specificity and simplicity for CA125 detection, but they suffer from strong interference from autofluorescence of biosamples. To resolve this problem, near-infrared (NIR)-excitable upconversion nanoparticles (UCNPs) was employed as energy donor to fabricate CA125 biosensor. Carbon dots (CDs) were utilized as energy acceptor for the overlap between the upconversion luminescence (UCL) spectrum of UCNPs and the absorption spectrum of CDs. Aptamer-modified UCNPs were combined with CDs through π-π stacking interaction, which triggered the LRET process and induce UCL quenching with a high quenching efficiency of up to 90%. In presence of CA125, the formation of CA125-aptamer complex blocked the π-π stacking and recovered UCL. The UCL intensity increased linearly with the logarithm of CA125 concentration in the range from 0.01 to 100 U·mL−1. A detection limit of 9.0 × 10−3U·mL−1was obtained. This probe is also available for CA125 detection in human serum and may be a useful tool for the early detection of ovarian cancer.
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