Effects of spaceflight aboard the International Space Station on mouse estrous cycle and ovarian gene expression.
Effects of spaceflight aboard the International Space Station on mouse estrous cycle and ovarian gene expression.
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国际空间站航天飞行对小鼠发情周期和卵巢基因表达的影响。
DOI：
10.1038/s41526-021-00139-7
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发表时间：
2021-03-12
期刊：
影响因子：
5.1
通讯作者：
Christenson LK
中科院分区：
文献类型：
--
作者：
Hong X;Ratri A;Choi SY;Tash JS;Ronca AE;Alwood JS;Christenson LK
卵巢类固醇显著影响体内大多数组织的正常稳态和代谢过程，包括肌肉、骨骼、神经、免疫、心血管和生殖系统。因此，确定航天飞行对卵巢和发情周期的影响对于我们理解所有使用雌性小鼠的航天实验至关重要。成年雌性小鼠（n = 10）在微重力空间飞行37天后在轨暴露并被处死。当代对照组（飞行前基线、饲养室和栖息地; n = 10/组）在处死前保存在肯尼迪航天中心，并在NASA艾姆斯研究中心进行类似的组织收集。在最初的尸体解冻时，收集卵巢组织并进行RNA和类固醇分析。将从两次冷冻/解冻的尸体中收集的阴道壁组织固定用于发情周期阶段测定。发情周期各阶段动物的比例（即，发情前期、发情期、发情后期和间情期）在基线、饲养室和飞行小鼠之间没有明显差异，而栖息地对照小鼠在间情期表现出更大的数量。卵巢组织类固醇浓度表明，雌二醇在各组之间没有差异，而孕酮水平较低（p < 0.05），在栖息地和飞行相比，基线女性。参与卵巢类固醇生成功能的基因在各组之间没有差异表达。由于卵巢雌激素可以显著影响多种非生殖组织，这些数据支持所有在太空飞行的雌性小鼠的阴道壁发情周期分类。此外，由于在不同的发情周期阶段观察到长期航天飞行的女性，这表明女性可能正在经历卵巢周期，并且可能具有生育能力。
Ovarian steroids dramatically impact normal homeostatic and metabolic processes of most tissues within the body, including muscle, bone, neural, immune, cardiovascular, and reproductive systems. Determining the effects of spaceflight on the ovary and estrous cycle is, therefore, critical to our understanding of all spaceflight experiments using female mice. Adult female mice (n = 10) were exposed to and sacrificed on-orbit after 37 days of spaceflight in microgravity. Contemporary control (preflight baseline, vivarium, and habitat; n = 10/group) groups were maintained at the Kennedy Space Center, prior to sacrifice and similar tissue collection at the NASA Ames Research Center. Ovarian tissues were collected and processed for RNA and steroid analyses at initial carcass thaw. Vaginal wall tissue collected from twice frozen/thawed carcasses was fixed for estrous cycle stage determinations. The proportion of animals in each phase of the estrous cycle (i.e., proestrus, estrus, metestrus, and diestrus) did not appreciably differ between baseline, vivarium, and flight mice, while habitat control mice exhibited greater numbers in diestrus. Ovarian tissue steroid concentrations indicated no differences in estradiol across groups, while progesterone levels were lower (p < 0.05) in habitat and flight compared to baseline females. Genes involved in ovarian steroidogenic function were not differentially expressed across groups. As ovarian estrogen can dramatically impact multiple non-reproductive tissues, these data support vaginal wall estrous cycle classification of all female mice flown in space. Additionally, since females exposed to long-term spaceflight were observed at different estrous cycle stages, this indicates females are likely undergoing ovarian cyclicity and may yet be fertile.
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影响因子：
3.5
作者：
Sanchez-Andrade, G.;Kendrick, K. M.
通讯作者：
Kendrick, K. M.
DOI：
10.1530/jrf.0.0270233
发表时间：
1971-01-01
期刊：
JOURNAL OF REPRODUCTION AND FERTILITY
影响因子：
--
作者：
CHAMPLIN, AK
通讯作者：
CHAMPLIN, AK
DOI：
10.1530/jrf.0.1090193
发表时间：
1997-03-01
期刊：
JOURNAL OF REPRODUCTION AND FERTILITY
影响因子：
--
作者：
Burden, HW;Zary, J;Hodson, CA
通讯作者：
Hodson, CA
影响因子：
4.8
作者：
Bertolin, Kalyne;Gossen, Jan;Murphy, Bruce D.
通讯作者：
Murphy, Bruce D.
影响因子：
4.6
作者：
Ronca, April E.;Moyer, Eric L.;Globus, Ruth K.
通讯作者：
Globus, Ruth K.