Production of hepatocyte-like cells from human pluripotent stem cells.
Production of hepatocyte-like cells from human pluripotent stem cells.
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DOI：
10.1038/nprot.2012.153
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发表时间：
2013-02
期刊：
影响因子：
14.8
通讯作者：
中科院分区：
文献类型：
--
作者：
大规模生产来自不同遗传背景的肝细胞将有利于药物筛选，并提供用作肝移植替代品的细胞来源。然而，完全功能的原代肝细胞仍然难以在体外扩增，通过使用替代细胞来源来解决这一问题是可取的。在这里，我们描述了一个25天的方案，以指导人类多能干细胞分化为接近同质的肝细胞样细胞群。当细胞在这个过程中进展时，它们表达基因的时间顺序与体内肝脏发育过程中描述的相似。该方案依赖于没有血清、喂食器或复杂细胞外基质的培养系统，能够在不受未知因素干扰的情况下进行分子分析。这种方法对人类胚胎干细胞和人类诱导多能干细胞有效，最近被用于体外肝脏疾病模型。
Large scale production of hepatocytes from a variety of genetic backgrounds would be beneficial for drug screening and to provide a source of cells to be used as a substitute for liver transplantation. However, fully functional primary hepatocytes remain difficult to expand in vitro and circumventing this problem by using an alternative source of cells is desirable. Here, we describe a 25 day protocol to direct the differentiation of human pluripotent stem cells into a near homogenous population of hepatocyte-like cells. As cells progress through this protocol they express genes in a chronological manner similar to that described during in-vivo hepatic development. The protocol relies on culture systems devoid of serum, feeders or complex extra-cellular matrices enabling molecular analyses without interference from unknown factors. This approach works efficiently with human embryonic stem cells and human induced pluripotent stem cells and was recently used to model liver diseases in vitro.
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影响因子：
64.8
作者：
Brons, I. Gabrielle M.;Smithers, Lucy E.;Vallier, Ludovic
通讯作者：
Vallier, Ludovic
影响因子：
56.9
作者：
Thomson, JA;Itskovitz-Eldor, J;Jones, JM
通讯作者：
Jones, JM
DOI：
10.1124/jpet.103.054841
发表时间：
2003-11-01
影响因子：
3.5
作者：
Stevens, JC;Hines, RN;Zaya, MJ
通讯作者：
Zaya, MJ
影响因子：
64.5
作者：
Takahashi, Kazutoshi;Tanabe, Koji;Yamanaka, Shinya
通讯作者：
Yamanaka, Shinya
影响因子：
5.2
作者：
Yamada, T;Yoshikawa, M;Tsunoda, Y
通讯作者：
Tsunoda, Y