Differential gene expression and protein abundance evince ontogenetic bias toward castes in a primitively eusocial wasp.
Differential gene expression and protein abundance evince ontogenetic bias toward castes in a primitively eusocial wasp.
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DOI：
10.1371/journal.pone.0010674
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发表时间：
2010-05-17
期刊：
影响因子：
3.7
通讯作者：
Amdam GV
中科院分区：
文献类型：
--
作者：
Hunt JH;Wolschin F;Henshaw MT;Newman TC;Toth AL;Amdam GV
Polistes纸黄蜂是理解可能具有工蜂和蜂王种姓起源特征的条件的模型，因此，纸黄蜂社会性的起源。波里斯特斯是“非正式的社会主义”，因为它具有依赖于上下文的种姓决定，并且种姓之间没有形态上的差异。即便如此，蜂蚁群也有一个时间模式，在这个时间模式中，由母蜂饲养的大多数雌性幼虫成为工蜂，而由工蜂饲养的大多数雌性幼虫成为未来生殖的雌蜂。这种模式被假设为反映了两种途径的发展，这可能利用机制，调节滞育在其他昆虫。使用表达序列标签（EST）的蜜蜂，我们选择了候选基因差异表达的其他昆虫在三个类别：1）滞育与非滞育表型和/或工人与女王分化，2）行为的工蜂的亚种级，和3）没有一个先验的期望在工蜂/雌蜂发展的作用。我们还使用了非靶向蛋白质组学筛选来测试肽/蛋白质丰度差异，这可以反映幼虫发育分化。我们发现，foundress-reared幼虫（假定的工作者注定）和工作者饲养的幼虫（假定的雌蕊注定）不同的16个基因的定量表达，其中12个与种姓和/或滞育在其他昆虫，他们也不同的丰富的9肽/蛋白质。一些差异表达的基因参与其他昆虫的滞育调节，其他差异表达的基因和蛋白质参与胰岛素信号通路，营养代谢和高度社会性蜜蜂的种姓决定。编码六聚体贮藏蛋白的基因和肽的差异表达尤其值得注意。虽然不是结论性的，但我们的结果支持以下假设：1）幼虫发育途径分歧可能导致成虫的种姓偏见，2）营养差异是途径分歧的基础。最后，在蜂幼虫的差异表达的基因和蛋白质，也差异表达在蜂王与工蜂种姓的发展可能表明，种姓结果的调节机制之间的相似性，reproductively eusocial和先进的eusocial Hyplittera。
Polistes paper wasps are models for understanding conditions that may have characterized the origin of worker and queen castes and, therefore, the origin of paper wasp sociality. Polistes is “primitively eusocial” by virtue of having context-dependent caste determination and no morphological differences between castes. Even so, Polistes colonies have a temporal pattern in which most female larvae reared by the foundress become workers, and most reared by workers become future-reproductive gynes. This pattern is hypothesized to reflect development onto two pathways, which may utilize mechanisms that regulate diapause in other insects. Using expressed sequence tags (ESTs) for Polistes metricus we selected candidate genes differentially expressed in other insects in three categories: 1) diapause vs. non-diapause phenotypes and/or worker vs. queen differentiation, 2) behavioral subcastes of worker honey bees, and 3) no a priori expectation of a role in worker/gyne development. We also used a non-targeted proteomics screen to test for peptide/protein abundance differences that could reflect larval developmental divergence. We found that foundress-reared larvae (putative worker-destined) and worker-reared larvae (putative gyne-destined) differed in quantitative expression of sixteen genes, twelve of which were associated with caste and/or diapause in other insects, and they also differed in abundance of nine peptides/proteins. Some differentially-expressed genes are involved in diapause regulation in other insects, and other differentially-expressed genes and proteins are involved in the insulin signaling pathway, nutrient metabolism, and caste determination in highly social bees. Differential expression of a gene and a peptide encoding hexameric storage proteins is especially noteworthy. Although not conclusive, our results support hypotheses of 1) larval developmental pathway divergence that can lead to caste bias in adults and 2) nutritional differences as the foundation of the pathway divergence. Finally, the differential expression in Polistes larvae of genes and proteins also differentially expressed during queen vs. worker caste development in honey bees may indicate that regulatory mechanisms of caste outcomes share similarities between primitively eusocial and advanced eusocial Hymenoptera.
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高度社会性蜜蜂意大利蜜蜂种姓分化的分子决定因素。
影响因子：
--
作者：
Barchuk AR;Cristino AS;Kucharski R;Costa LF;Simões ZL;Maleszka R
通讯作者：
Maleszka R
影响因子：
2.2
作者：
Hayward, SAL;Pavlides, SC;Denlinger, DL
通讯作者：
Denlinger, DL
影响因子：
--
作者：
Gobbi, Nivar;Noll, Fernando B.;Penna, Marcelo A. H.
通讯作者：
Penna, Marcelo A. H.
影响因子：
2.2
作者：
GADAGKAR, R;BHAGAVAN, S;VINUTHA, C
通讯作者：
VINUTHA, C
DOI：
10.1073/pnas.0333979100
发表时间：
2003-02-18
影响因子：
11.1
作者：
Amdam, GV;Norberg, K;Omholt, SW
通讯作者：
Omholt, SW