Preliminary quantitative profile of differential protein expression between rat L6 myoblasts and myotubes by stable isotope labeling with amino acids in cell culture.
Preliminary quantitative profile of differential protein expression between rat L6 myoblasts and myotubes by stable isotope labeling with amino acids in cell culture.
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通过细胞培养物中氨基酸的稳定同位素标记对大鼠 L6 成肌细胞和肌管之间差异蛋白表达的初步定量分析
DOI：
10.1002/pmic.200800354
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发表时间：
2009-03
期刊：
影响因子：
3.4
通讯作者：
Yang, Fuquan
中科院分区：
文献类型：
--
作者：
Cui, Ziyou;Chen, Xiulan;Lu, Bingwen;Park, Sung Kyu;Xu, Tao;Xie, Zhensheng;Xue, Peng;Hou, Junjie;Hang, Haiying;Yates, John R., III;Yang, Fuquan
近年来，对肌生成机制的研究取得了进展。骨骼肌分化是一个多步骤的过程，在转录水平上受多种因子的时空调控。为了探索这些因素参与肌形成，稳定同位素标记与细胞培养中的氨基酸（SILAC），加上高精度质谱（LTQ-Orbitrap），成功地应用。大鼠L 6细胞系是研究体外肌肉成肌的理想模型。当单核的L 6成肌细胞在培养板中达到汇合时，血清饥饿可使其转化为多核的肌管。通过比较L 6成肌细胞和终末分化的多核肌管的蛋白质表达，1170个蛋白质被定量，379个蛋白质在完全分化的肌管中与成肌细胞相比发生显著变化。这些差异表达的蛋白质主要涉及细胞内或细胞间信号传导、蛋白质合成和降解、蛋白质折叠、细胞粘附和细胞外基质、细胞结构和运动、代谢、物质转运等。其中许多上调的蛋白在本研究中首次被发现参与促进骨骼肌分化。总之，我们的研究结果为了解肌细胞发生的机制提供了新的线索。
Defining the mechanisms governing myogenesis has advanced in recent years. Skeletal-muscle differentiation is a multi-step process controlled spatially and temporally by various factors at the transcription level. To explore those factors involved in myogenesis, stable isotope labeling with amino acids in cell culture (SILAC), coupled with high accuracy mass spectrometry (LTQ-Orbitrap), was applied successfully. Rat L6 cell line is an excellent model system for studying muslce myogenesis in vitro. When mononucleate L6 myoblast cells reach confluent in culture plate, they could transform into multinucleate myotubes by serum starvation. By comparing protein expression of L6 myoblasts and terminally differentiated multinucleated myotubes, 1170 proteins were quantified and 379 proteins changed significantly in fully differentiated myotubes in contrast to myoblasts. These differentially expressed proteins are mainly involved in inter-or intracellular signaling, protein synthesis and degradation, protein folding, cell adhesion and extracelluar matrix, cell structure and motility, metabolism, substance transportation, etc. These findings were supported by many previous studies on myogenic differentiation, of which many up-regulated proteins were found to be involved in promoting skeletal muscle differentiation for the first time in our study. In sum, our results provide new clues for understanding the mechanism of myogenesis.
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前列腺素F2（α）通过NFATC2依赖性途径刺激骨骼肌细胞的生长。
DOI：
10.1083/jcb.200208085
发表时间：
2003-04-14
期刊：
The Journal of cell biology
影响因子：
--
作者：
Horsley V;Pavlath GK
通讯作者：
Pavlath GK
影响因子：
56.9
作者：
Johnson, GL;Lapadat, R
通讯作者：
Lapadat, R
影响因子：
4.8
作者：
Bondesen, Brenda A.;Jones, Kristen A.;Pavlath, Grace K.
通讯作者：
Pavlath, Grace K.
影响因子：
4
作者：
Kessels, MM;Dong, JX;Qualmann, B
通讯作者：
Qualmann, B
影响因子：
3.4
作者：
Gonnet, Florence;Bouazza, Belaid;Svinartchouk, Fedor
通讯作者：
Svinartchouk, Fedor