Persistence of viral DNA in the epithelial basal layer suggests a model for papillomavirus latency following immune regression.
Persistence of viral DNA in the epithelial basal layer suggests a model for papillomavirus latency following immune regression.
复制标题
DOI：
10.1016/j.virol.2011.03.019
复制
发表时间：
2011-06-05
期刊：
影响因子：
3.7
通讯作者：
Doorbar J
中科院分区：
文献类型：
--
作者：
Maglennon GA;McIntosh P;Doorbar J
兔口腔乳头瘤病毒（ROPV）可引起兔的良性和自发消退的口腔病变，是与低风险人乳头瘤病毒类型相关的疾病的有用模型。在这里，我们已经调整了ROPV系统研究乳头瘤病毒的潜伏期。病变消退后，ROPV DNA在大多数消退部位持续存在，其水平显著低于在生产性乳头状瘤中发现的水平。还检测到剪接的病毒转录物。在感染和病变消退后，可以证明在无疾病的情况下ROPV持续存在一年。这与病毒生命周期的完成或新病毒体的产生无关，表明ROPV持续处于潜伏状态。使用新的激光捕获显微切割技术，我们可以表明，该网站的潜伏期是一个子集的基底上皮细胞在以前的实验感染的网站。我们假设这些细胞是上皮干细胞，潜伏期的重新激活可能是疾病复发的一个来源。
Rabbit oral papillomavirus (ROPV) causes benign and spontaneously regressing oral lesions in rabbits, and is a useful model of disease associated with low-risk human papillomavirus types. Here we have adapted the ROPV system to study papillomavirus latency. Following lesion regression, ROPV DNA persists at the majority of regressed sites at levels substantially lower than those found in productive papillomas. Spliced viral transcripts were also detected. ROPV persistence in the absence of disease could be demonstrated for a year following infection and lesion-regression. This was not associated with completion of the virus life-cycle or new virion production, indicating that ROPV persists in a latent state. Using novel laser capture microdissection techniques, we could show that the site of latency is a subset of basal epithelial cells at sites of previous experimental infection. We hypothesize that these cells are epithelial stem cells and that reactivation of latency may be a source of recurrent disease.
登录
查看更多内容
影响因子：
1.4
作者：
PIGNATARI, S;SMITH, EM;TUREK, LP
通讯作者：
TUREK, LP
影响因子：
158.5
作者：
STEINBERG, BM;TOPP, WC;ABRAMSON, AL
通讯作者：
ABRAMSON, AL
影响因子：
5.4
作者：
Embers, ME;Budgeon, LR;Christensen, ND
通讯作者：
Christensen, ND
影响因子：
3.7
作者：
Kalantari, Mina;Garcia-Carranca, Alejandro;Dalia Morales-Vazquez, Claudia;Zuna, Rosemary;Perez Montiel, Delia;Calleja-Macias, Itzel E.;Johansson, Bo;Andersson, Sonia;Bernard, Hans-Ulrich
通讯作者：
Bernard, Hans-Ulrich
影响因子：
5.4
作者：
Oh, ST;Longworth, MS;Laimins, LA
通讯作者：
Laimins, LA