Plasma levels of leptin, omentin, collagenous repeat-containing sequence of 26-kDa protein (CORS-26) and adiponectin before and after oral glucose uptake in slim adults.
Plasma levels of leptin, omentin, collagenous repeat-containing sequence of 26-kDa protein (CORS-26) and adiponectin before and after oral glucose uptake in slim adults.
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DOI：
10.1186/1475-2840-6-7
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发表时间：
2007-02-20
影响因子：
9.3
通讯作者：
Buechler C
中科院分区：
文献类型：
--
作者：
Wurm S;Neumeier M;Weigert J;Schäffler A;Buechler C
脂肪组织分泌蛋白统称为脂肪细胞因子，包括瘦素、脂联素、抵抗素、含有胶原重复序列的 26-kDa 蛋白 (CORS-26) 和网膜蛋白。其中一些脂肪细胞因子影响胰岛素敏感性和葡萄糖代谢，因此全身水平可能受到口服葡萄糖摄取的影响。尽管瘦素和脂联素的研究结果相互矛盾，但抵抗素尚未得到广泛研究，也没有关于网膜素和 CORS-26 的报道。因此，通过 ELISA 或免疫印迹分析了 20 名高度胰岛素敏感的年轻人在口服葡萄糖负荷之前和之后 120 分钟这些蛋白质的血浆水平。摄入葡萄糖后 2 小时，循环瘦素减少，而脂联素和抵抗素水平没有变化。葡萄糖摄入前后脂联素和 CORS-26 亚型的分布相似。网膜蛋白在血浆中含量很高，免疫印迹分析显示，比较葡萄糖摄入前和摄入后 2 小时后的血浆水平没有变化。总而言之，我们的数据表明，在胰岛素敏感先证者中，只有瘦素因葡萄糖摄取而减少，而脂联素和抵抗素则没有改变。 CORS-26 首次被证明以高分子量形式在血浆中循环，并且与网膜蛋白一样不受口服葡萄糖负荷的影响。网膜素被证明可以增强胰岛素刺激的葡萄糖摄取，但全身水平与餐后血糖无关。
Adipose tissue secreted proteins are collectively named adipocytokines and include leptin, adiponectin, resistin, collagenous repeat-containing sequence of 26-kDa protein (CORS-26) and omentin. Several of these adipocytokines influence insulin sensitivity and glucose metabolism and therefore systemic levels may be affected by oral glucose uptake. Whereas contradictory results have been published for leptin and adiponectin, resistin has not been extensively investigated and no reports on omentin and CORS-26 do exist. Therefore the plasma levels of these proteins before and 120 min after an oral glucose load were analyzed in 20 highly-insulin sensitive, young adults by ELISA or immunoblot. Circulating leptin was reduced 2 h after glucose uptake whereas adiponectin and resistin levels are not changed. Distribution of adiponectin and CORS-26 isoforms were similar before and after glucose ingestion. Omentin is highly abundant in plasma and immunoblot analysis revealed no alterations when plasma levels before and 2 h after glucose intake were compared. Taken together our data indicate that only leptin is reduced by glucose uptake in insulin-sensitive probands whereas adiponectin and resistin are not altered. CORS-26 was demonstrated for the first time to circulate as high molecular weight form in plasma and like omentin was not influenced by oral glucose load. Omentin was shown to enhance insulin-stimulated glucose uptake but systemic levels are not correlated to postprandial blood glucose.
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DOI：
10.1016/j.bbaexp.2005.11.005
发表时间：
2005-12-30
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
影响因子：
--
作者：
Schäffler, A;Neumeier, A;Büchler, C
通讯作者：
Büchler, C
影响因子：
3.2
作者：
Corica, F;Corsonello, A;Buemi, M
通讯作者：
Buemi, M
DOI：
10.1152/ajpendo.00572.2004
发表时间：
2006-06-01
影响因子：
5.1
作者：
Yang, Rong-Ze;Lee, Mi-Jeong;Gong, Da-Wei
通讯作者：
Gong, Da-Wei
影响因子：
4.8
作者：
Pajvani, UB;Hawkins, M;Scherer, PE
通讯作者：
Scherer, PE
影响因子：
5.5
作者：
Neumeier, Markus;Weigert, Johanna;Buechler, Christa
通讯作者：
Buechler, Christa