Intracellular calcium signal at the leading edge regulates mesodermal sheet migration during Xenopus gastrulation.
Intracellular calcium signal at the leading edge regulates mesodermal sheet migration during Xenopus gastrulation.
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DOI：
10.1038/s41598-018-20747-w
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发表时间：
2018-02-05
影响因子：
4.6
通讯作者：
Ueno N
中科院分区：
文献类型：
--
作者：
Hayashi K;Yamamoto TS;Ueno N
在动物胚胎发生的原肠胚形成阶段，引导轴向中胚层的细胞向胚胎的前侧迁移，有力地延伸细胞突起，如板状伪足。据认为，引导细胞感觉到从外胚层细胞发出的化学引诱物的梯度，并翻译它们以启动和维持原肠胚形成所必需的细胞运动。然而，目前尚不清楚细胞外信息如何转化为导致运动的细胞内化学反应。在这里，我们证明了细胞内的Ca 2+水平的形成的领先细胞显着高于以下的细胞和轴向中胚层细胞。我们还发现，抑制细胞内Ca 2+显着延缓原肠胚细胞的运动，而增加细胞内Ca 2+与离子载体增强迁移。我们进一步发现，离子载体处理增加了这些细胞中小GTdR Rac 1的活性形式。我们的研究结果表明，瞬时胞内Ca 2+信号在原肠胚形成过程中的细胞迁移活动中起着至关重要的作用。
During the gastrulation stage in animal embryogenesis, the cells leading the axial mesoderm migrate toward the anterior side of the embryo, vigorously extending cell protrusions such as lamellipodia. It is thought that the leading cells sense gradients of chemoattractants emanating from the ectodermal cells and translate them to initiate and maintain the cell movements necessary for gastrulation. However, it is unclear how the extracellular information is converted to the intracellular chemical reactions that lead to motion. Here we demonstrated that intracellular Ca2+ levels in the protrusion-forming leading cells are markedly higher than those of the following cells and the axial mesoderm cells. We also showed that inhibiting the intracellular Ca2+ significantly retarded the gastrulation cell movements, while increasing the intracellular Ca2+ with an ionophore enhanced the migration. We further found that the ionophore treatment increased the active form of the small GTPase Rac1 in these cells. Our results suggest that transient intracellular Ca2+ signals play an essential role in the active cell migration during gastrulation.
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影响因子：
64.5
作者：
Cheung KJ;Gabrielson E;Werb Z;Ewald AJ
通讯作者：
Ewald AJ
影响因子：
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Marsden, M;DeSimone, DW
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DeSimone, DW
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64.5
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Guan, Chen-bing;Xu, Hua-tai;Poo, Mu-ming
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Poo, Mu-ming
影响因子：
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作者：
Markova O;Sénatore S;Chardès C;Lenne PF
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Lenne PF
影响因子：
4.6
作者：
Morita, Hitoshi;Kajiura-Kobayashi, Hiroko;Ueno, Naoto
通讯作者：
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