Decreased and dysfunctional circulating endothelial progenitor cells in patients with chronic obstructive pulmonary disease
Decreased and dysfunctional circulating endothelial progenitor cells in patients with chronic obstructive pulmonary disease
复制标题
慢性阻塞性肺病患者循环内皮祖细胞减少和功能障碍
DOI：
10.3760/cma.j.issn.0366-6999.20122633
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发表时间：
2013-09
期刊：
影响因子：
--
通讯作者：
CHEN Ping
中科院分区：
文献类型：
--
作者：
CAI Shan;XIANG Xu-dong;ZHOU Rui;CHEN Ping
背景：已有研究表明内皮祖细胞参与多种疾病的发生，并具有治疗意义。为了明确慢性阻塞性肺疾病肺血管内环境的改变，我们试图观察慢性阻塞性肺疾病患者循环内皮祖细胞的水平和功能。方法研究对象为慢性阻塞性肺疾病患者20例，对照组20例。流式细胞仪计数外周血内皮祖细胞(CD34+/CD133+/VEGFR-2+细胞)。体外培养循环内皮祖细胞，通过摄取DiIacLDL，结合UEA-I，表达von Willebrand因子和内皮型一氧化氮合酶。通过检测慢性阻塞性肺疾病患者外周血中内皮祖细胞的黏附、增殖、一氧化氮的产生以及内皮型一氧化氮合酶和磷酸化内皮型一氧化氮合酶的表达来检测循环内皮祖细胞功能。结果慢性阻塞性肺疾病组外周血内皮祖细胞数量低于对照组，分别为(0.54±0.16)%和(1.15±0.57)%，P<0.05。在体外培养的贴壁单个核细胞中，约有80%的细胞表达Dil-acLDL和UEA-1，其中92%的细胞表达von Willebrand因子，91%的细胞表达内皮型一氧化氮合酶。与对照组比较，慢性阻塞性肺病患者外周血内皮祖细胞贴壁率为18.7±4.8个/视野，明显低于对照组的45.0±5.9个/视野，P<0.05。增殖实验显示慢性阻塞性肺疾病患者外周血内皮祖细胞的增殖能力明显受损：0.135±0.038比0.224±0.042，P<0.05。慢性阻塞性肺疾病组内皮细胞一氧化氮合酶活性(112.06±10.00vs.135.41±5.38，P<0.05)、磷酸化内皮型一氧化氮合酶蛋白表达(88.89±4.98vs.117.98±16.49，P<0.05)、一氧化氮生成量((25.11±5.27)mgr；摩尔/L比(37.72±7.10)&mgr；摩尔/L，P<0.05)均明显低于对照组。结论慢性阻塞性肺疾病患者外周血内皮祖细胞减少，功能受损。
Background It has been widely demonstrated that endothelial progenitor cells are involved in several diseases and that they have therapeutic implications. In order to define the altered pulmonary vascular homeostasis in chronic obstructive pulmonary disease, we sought to observe the level and functions of circulating endothelial progenitor cells in patients with chronic obstructive pulmonary disease. Methods The total study population included 20 patients with chronic obstructive pulmonary disease and 20 control subjects. The number of circulating endothelial progenitor cells (CD34+/CD133+/VEGFR‐2+ cells) was counted by flow cytometry. Circulating endothelial progenitor cells were also cultured in vitro and characterized by uptake of DiIacLDL, combining with UEA‐I, and expression of von Willebrand factor and endothelial nitric oxide synthase. Adhesion, proliferation, production of nitric oxide, and expression of endothelial nitric oxide synthase and phosphorylated‐endothelial nitric oxide synthase were detected to determine functions of circulating endothelial progenitor cells in patients with chronic obstructive pulmonary disease. Results The number of circulating endothelial progenitor cells in the chronic obstructive pulmonary disease group was lower than in the control group: (0.54±0.16)% vs. (1.15±0.57)%, P <0.05. About 80% of adherent peripheral blood mononuclear cells cultured in vitro were double labeled with Dil‐acLDL and UEA‐1. The 92% and 91% of them were positive for von Willebrand factor and endothelial nitric oxide synthase, respectively. Compared with the control, there were significantly fewer adhering endothelial progenitor cells in chronic obstructive pulmonary disease patients: 18.7±4.8/field vs. 45.0±5.9/field, P <0.05. The proliferation assay showed that the proliferative capacity of circulating endothelial progenitor cells from chronic obstructive pulmonary disease patients was significantly impaired: 0.135±0.038 vs. 0.224±0.042, P <0.05. Furthermore, nitric oxide synthase (112.06±10.00 vs. 135.41±5.38, P <0.05), phosphorylated endothelial nitric oxide synthase protein expression (88.89±4.98 vs. 117.98±16.49, P <0.05) and nitric oxide production ((25.11±5.27) &mgr;mol/L vs. (37.72±7.10) &mgr;mol/L, P <0.05) were remarkably lower in endothelial cells from the chronic obstructive pulmonary disease group than the control. Conclusion Circulating endothelial progenitor cells were decreased and functionally impaired in patients with chronic obstructive pulmonary disease.
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影响因子：
20.1
作者：
Vasa, M;Fichtlscherer, S;Dimmeler, S
通讯作者：
Dimmeler, S
影响因子：
5.5
作者：
Grisar, J.;Steiner, C. W.;Smolen, J. S.
通讯作者：
Smolen, J. S.
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影响因子：
6.9
作者：
Cai, Shan;Chen, Ping;Wu, Jie
通讯作者：
Wu, Jie
影响因子：
2.6
作者：
Voelkel NF;Gomez-Arroyo J;Mizuno S
通讯作者：
Mizuno S
影响因子：
6.9
作者：
Takahashi, Toru;Suzuki, Satoshi;Kato, Masato
通讯作者：
Kato, Masato