Characterization of the rapid transcriptional response to long-term sensitization training in Aplysia californica.
Characterization of the rapid transcriptional response to long-term sensitization training in Aplysia californica.
复制标题
对加州阿普利亚的长期致敏训练的快速转录反应的表征。
DOI：
10.1016/j.nlm.2014.07.009
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发表时间：
2014-12
影响因子：
2.7
通讯作者：
Calin-Jageman, Robert J.
中科院分区：
文献类型：
--
作者：
Herdegen, Samantha;Holmes, Geraldine;Cyriac, Ashly;Calin-Jageman, Irina E.;Calin-Jageman, Robert J.
我们使用了定制设计的微阵列和定量PCR来表征的快速转录反应，长期的敏化训练的海洋软体动物加州滨对虾。失智症患者暴露于身体一侧的重复伤害性电击，这是一种已知的诱导长期的、转录依赖性的反射反应性增加的程序，仅限于训练一侧。训练后1小时，从训练和未训练的身体侧收获胸膜神经节;这些神经节含有有助于介导长期敏化记忆表达的感觉伤害感受器。来自8个生物重复的微阵列分析表明，长期敏化训练快速调节至少81个转录本。我们使用qPCR测试了这些转录本的一个子集，发现83%在相同的样本中得到证实，其中86%在独立样本中再次得到证实。因此，我们的新的微阵列设计显示出强大的收敛和预测的有效性，用于分析记忆的转录相关的记忆在Aaplasia。完全验证的转录本包括一些以前确定为在这种范式（ApC/EBP和ApEgr）中调节的转录本，但也包括新的发现。具体来说，我们表明，长期敏化训练迅速上调转录本的表达，这些转录本可能编码一种C/EBPγ转录因子、一种甘氨酸转运蛋白（GlyT 2）和一种空泡蛋白分选相关蛋白（VPS 36）的失智症同源物。
We used a custom-designed microarray and quantitative PCR to characterize the rapid transcriptional response to long-term sensitization training in the marine mollusk Aplysia californica. Aplysia were exposed to repeated noxious shocks to one side of the body, a procedure known to induce a longlasting, transcription-dependent increase in reflex responsiveness that is restricted to the side of training. One hour after training, pleural ganglia from the trained and untrained sides of the body were harvested; these ganglia contain the sensory nociceptors which help mediate the expression of longterm sensitization memory. Microarray analysis from 8 biological replicates suggests that long-term sensitization training rapidly regulates at least 81 transcripts. We used qPCR to test a subset of these transcripts and found that 83% were confirmed in the same samples, and 86% of these were again confirmed in an independent sample. Thus, our new microarray design shows strong convergent and predictive validity for analyzing the transcriptional correlates of memory in Aplysia. Fully validated transcripts include some previously identified as regulated in this paradigm (ApC/EBP and ApEgr) but also include novel findings. Specifically, we show that long-term sensitization training rapidly upregulates the expression of transcripts which may encode Aplysia homologs of a C/EBPγ transcription factor, a glycine transporter (GlyT2), and a vacuolar-protein-sorting-associated protein (VPS36).
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影响因子：
4.4
作者：
Kadakkuzha BM;Akhmedov K;Capo TR;Carvalloza AC;Fallahi M;Puthanveettil SV
通讯作者：
Puthanveettil SV
影响因子：
12.3
作者：
Gentleman RC;Carey VJ;Bates DM;Bolstad B;Dettling M;Dudoit S;Ellis B;Gautier L;Ge Y;Gentry J;Hornik K;Hothorn T;Huber W;Iacus S;Irizarry R;Leisch F;Li C;Maechler M;Rossini AJ;Sawitzki G;Smith C;Smyth G;Tierney L;Yang JY;Zhang J
通讯作者：
Zhang J
影响因子：
2.7
作者：
Hermitte, G;Pedreira, ME;Maldonado, H
通讯作者：
Maldonado, H
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
3
作者：
Esdin, Joseph;Pearce, Kaycey;Glanzman, David L.
通讯作者：
Glanzman, David L.