All-in-One Optofluidic Chip for Molecular Biosensing Assays.
All-in-One Optofluidic Chip for Molecular Biosensing Assays.
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DOI：
10.3390/bios12070501
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发表时间：
2022-07-09
期刊：
影响因子：
--
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集成的生物传感器平台已经成为高度关注的主题，用于统一的测定制备和分析，以减少样品到应答的响应时间。通过将尽可能多的生物传感器过程和功能紧凑地组合到单个芯片实验室设备中，一体化护理点设备由于其紧凑的尺寸和便携性，可以帮助提高部署的可访问性和速度。生物标志物测定制备和感测是通常在单独的设备上进行的功能，因此增加了污染、样品体积损失和其他形式的错误的机会。在这里，我们展示了一个完整的芯片实验室系统，结合样品制备，芯片上的光流体染料激光器和光学检测。首先，我们展示了集成的芯片上的分布式反馈染料激光器的无干扰的光激发的粒子移动通过流体通道。通过使用罗丹明6G作为增益介质在575 nm处激发单个荧光微球来证明这种能力。接下来，我们提出了一个结合微阀网络（自动机）的样品制备纳升体积，芯片上的分布式反馈染料激光器的目标激发，和光学检测的光流体PDMS平台。我们在30分钟内对磁珠上的寨卡病毒核酸进行同步捕获和荧光标记。然后，当载靶磁珠流过分析通道时，使用片上激光器对其进行光学激发，然后进行高度特异性的荧光检测。这种完整的一体化生物传感器的演示是开发快速，即时护理设备的切实步骤，可以帮助限制未来爆发的严重程度。
Integrated biosensor platforms have become subjects of high interest for consolidated assay preparation and analysis to reduce sample-to-answer response times. By compactly combining as many biosensor processes and functions as possible into a single lab-on-chip device, all-in-one point-of-care devices can aid in the accessibility and speed of deployment due to their compact size and portability. Biomarker assay preparation and sensing are functionalities that are often carried out on separate devices, thus increasing opportunity of contamination, loss of sample volume, and other forms of error. Here, we demonstrate a complete lab-on-chip system combining sample preparation, on-chip optofluidic dye laser, and optical detection. We first show the integration of an on-chip distributed feedback dye laser for alignment-free optical excitation of particles moving through a fluidic channel. This capability is demonstrated by using Rhodamine 6G as the gain medium to excite single fluorescent microspheres at 575 nm. Next, we present an optofluidic PDMS platform combining a microvalve network (automaton) for sample preparation of nanoliter volumes, on-chip distributed feedback dye laser for target excitation, and optical detection. We conduct concurrent capture and fluorescence tagging of Zika virus nucleic acid on magnetic beads in 30 min. Target-carrying beads are then optically excited using the on-chip laser as they flow through an analysis channel, followed by highly specific fluorescence detection. This demonstration of a complete all-in-one biosensor is a tangible step in the development of a rapid, point-of-care device that can assist in limiting the severity of future outbreaks.
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DOI：
10.1016/b978-0-12-813855-7.00013-1
发表时间：
2018-01-01
期刊：
NANOTECHNOLOGY AND BIOSENSORS
影响因子：
--
作者：
Nikoleli, Georgia-Paraskevi;Siontorou, Christina G.;Tzamtzis, Nikolaos
通讯作者：
Tzamtzis, Nikolaos
影响因子：
3.6
作者：
Sun, Yuze;Suter, Jonathan D.;Fan, Xudong
通讯作者：
Fan, Xudong
影响因子：
6.1
作者：
Szydzik, C.;Fernandez Gavela, A.;Lechuga, L. M.
通讯作者：
Lechuga, L. M.
DOI：
10.3390/s151229783
发表时间：
2015-12-01
期刊：
Sensors (Basel, Switzerland)
影响因子：
--
作者：
Luka G;Ahmadi A;Najjaran H;Alocilja E;DeRosa M;Wolthers K;Malki A;Aziz H;Althani A;Hoorfar M
通讯作者：
Hoorfar M
影响因子：
3.8
作者：
Li, ZY;Zhang, ZY;Psaltis, D
通讯作者：
Psaltis, D