Determination of cellular lipids bound to human CD1d molecules.
Determination of cellular lipids bound to human CD1d molecules.
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DOI：
10.1371/journal.pone.0005325
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发表时间：
2009
期刊：
影响因子：
3.7
通讯作者：
Hildebrand W
中科院分区：
文献类型：
--
作者：
Cox D;Fox L;Tian R;Bardet W;Skaley M;Mojsilovic D;Gumperz J;Hildebrand W
CD1分子是一种糖蛋白，它在细胞表面呈现脂质抗原，供特定的T淋巴细胞群体免疫识别。先前的实验数据表明，多种脂质物种可以与CD1分子结合，但对选择用于呈现的细胞配体的特性知之甚少。在这里，我们已经分子表征脂结合到人类CD1d同种异构体。从分泌的CD1d分子中洗脱配体，用正相高效液相色谱法分离配体，并用质谱法进行表征。共鉴定出177种脂类，包括甘油磷脂和鞘脂。甘油磷脂包括常见的二酰基甘油种类、被称为缩醛原的还原形式、溶磷脂（单酰基种类）和心磷脂（四酰基种类）。鞘脂包括鞘磷脂和糖基化形式，如神经节苷脂GM3。这些结果表明，人类CD1d分子在分泌途径中结合了令人惊讶的多种脂质结构，包括已报道在癌症、自身免疫性疾病、脂质信号传导和细胞死亡中发挥作用的化合物。
CD1 molecules are glycoproteins that present lipid antigens at the cell surface for immunological recognition by specialized populations of T lymphocytes. Prior experimental data suggest a wide variety of lipid species can bind to CD1 molecules, but little is known about the characteristics of cellular ligands that are selected for presentation. Here we have molecularly characterized lipids bound to the human CD1d isoform. Ligands were eluted from secreted CD1d molecules and separated by normal phase HPLC, then characterized by mass spectroscopy. A total of 177 lipid species were molecularly identified, comprising glycerophospholipids and sphingolipids. The glycerophospholipids included common diacylglycerol species, reduced forms known as plasmalogens, lyso-phospholipids (monoacyl species), and cardiolipins (tetraacyl species). The sphingolipids included sphingomyelins and glycosylated forms, such as the ganglioside GM3. These results demonstrate that human CD1d molecules bind a surprising diversity of lipid structures within the secretory pathway, including compounds that have been reported to play roles in cancer, autoimmune diseases, lipid signaling, and cell death.
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影响因子：
15
作者：
Goff, RD;Gao, Y;Savage, PB
通讯作者：
Savage, PB
影响因子：
4.4
作者：
Giabbai, B;Sidobre, SP;Degano, M
通讯作者：
Degano, M
DOI：
10.1016/s0021-9673(01)92081-3
发表时间：
1977-01-01
期刊：
JOURNAL OF CHROMATOGRAPHY
影响因子：
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作者：
HAX, WMA;GEURTSVANKESSEL, WSM
通讯作者：
GEURTSVANKESSEL, WSM
影响因子：
4.4
作者：
Hickman, HD;Luis, AD;Hildebrand, WH
通讯作者：
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DOI：
10.1016/j.jasms.2005.11.019
发表时间：
2006-03-01
影响因子：
3.2
作者：
Hsu, FF;Turk, J
通讯作者：
Turk, J