Isotope ratio monitoring of small molecules and macromolecules by liquid chromatography coupled to isotope ratio mass spectrometry.
Isotope ratio monitoring of small molecules and macromolecules by liquid chromatography coupled to isotope ratio mass spectrometry.
复制标题
通过液相色谱与同位素比质谱联用对小分子和大分子进行同位素比监测。
DOI：
10.1002/rcm.2117
复制
发表时间：
2005
期刊：
影响因子：
--
通讯作者：
G. Hopfgartner
中科院分区：
文献类型：
--
作者：
J. Godin;J. Hau;L. Fay;G. Hopfgartner
在同位素比质谱领域，液相色谱（LC）和同位素比质谱（IRMS）接口的引入开辟了一系列新的前景。LC界面基于化学氧化，从有机分子产生CO2。虽然第一个结果是从低分子量化合物的分析中获得的，但本文介绍了通过irm-LC/MS对其他分子，特别是生物分子进行化合物特异性同位素分析的应用。LC流速对CO2信号和对所观察到的delta 13 C值的影响被证明。血管紧张素III和亮氨酸的定量限分别为100和38 pmol，delta 13 C值的标准差优于0.4 ‰。此外，对元素分析仪-IRMS和流动注射分析-IRMS（FIA-LC/MS）的δ 13 C值的准确度和精密度进行了比较。对于分子量范围为131至66，390 Da的化合物，精密度优于千分之0.3，准确度为千分之0.1至千分之0.7。在工作的第二部分，一个二维（2D）-LC方法分离的15个underivatised氨基酸的证明，delta 13 C值的精度为几个氨基酸的irm-LC/MS是优于0.3千分之一的天然丰度。对于标记的混合物，变异系数为1%之间的0.07原子%过量（APE）的苏氨酸和丙氨酸，约10%的0.03 APE的缬氨酸和苯丙氨酸。IRM-LC/MS测定大鼠结肠粘膜提取物中13 C-苏氨酸的同位素富集的应用证明了0.5 ‰或0.001原子%的精度。
In the field of isotope ratio mass spectrometry, the introduction of an interface allowing the connection of liquid chromatography (LC) and isotope ratio mass spectrometry (IRMS) has opened a range of new perspectives. The LC interface is based on a chemical oxidation, producing CO2 from organic molecules. While first results were obtained from the analysis of low molecular weight compounds, the application of compound-specific isotope analysis by irm-LC/MS to other molecules, in particular biomolecules, is presented here. The influence of the LC flow rate on the CO2 signal and on the observed delta13C values is demonstrated. The limits of quantification for angiotensin III and for leucine were 100 and 38 pmol, respectively, with a standard deviation of the delta13C values better than 0.4 per thousand. Also, accuracy and precision of delta13C values for elemental analyser-IRMS and flow injection analysis-IRMS (FIA-LC/MS) were compared. For compounds with molecular weights ranging from 131 to 66,390 Da, precision was better than 0.3 per thousand, and accuracy varied from 0.1 to 0.7 per thousand. In a second part of the work, a two-dimensional (2D)-LC method for the separation of 15 underivatised amino acids is demonstrated; the precision of delta13C values for several amino acids by irm-LC/MS was better than 0.3 per thousand at natural abundance. For labelled mixtures, the coefficient of variation was between 1% at 0.07 atom % excess (APE) for threonine and alanine, and around 10% at 0.03 APE for valine and phenylalanine. The application of irm-LC/MS to the determination of the isotopic enrichment of 13C-threonine in an extract of rat colon mucosa demonstrated a precision of 0.5 per thousand, or 0.001 atom %.
登录
查看更多内容
使用化学反应接口并引入气相色谱或液相色谱进行连续流同位素比质谱分析。
影响因子：
7.4
作者：
Teffera,Y;Kusmierz,JJ;Abramson,FP
通讯作者：
Abramson,FP
用于非衍生化亮氨酸纯化的高效液相色谱技术：碳同位素分馏的证据。
DOI：
10.1016/0378-4347(92)80467-5
发表时间：
1992
期刊：
Journal of chromatography
影响因子：
--
作者：
Baumann,PQ;Ebenstein,DB;O'Rourke,BD;Nair,KS
通讯作者：
Nair,KS
使用化学反应界面质谱进行高效液相色谱的元素和同位素特异性检测。
DOI：
10.1016/0021-9673(95)01279-6
发表时间：
1996
期刊：
Journal of chromatography. A
影响因子：
--
作者：
McLean,M;Vestal,ML;Teffera,Y;Abramson,FP
通讯作者：
Abramson,FP
将非极性氨基酸还原为氨基醇，以增强高精度同位素分析的挥发性。
影响因子：
7.4
作者：
Zaideh,BI;Saad,NM;Lewis,BA;Brenna,JT
通讯作者：
Brenna,JT
影响因子：
7.4
作者：
GOODMAN, KJ;BRENNA, JT
通讯作者：
BRENNA, JT