Salmonella enterica Serovar Typhimurium Travels to Mesenteric Lymph Nodes Both with Host Cells and Autonomously.
Salmonella enterica Serovar Typhimurium Travels to Mesenteric Lymph Nodes Both with Host Cells and Autonomously.
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DOI：
10.4049/jimmunol.1701254
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发表时间：
2019-01-01
期刊：
影响因子：
--
通讯作者：
Milling SWF
中科院分区：
文献类型：
--
作者：
Bravo-Blas A;Utriainen L;Clay SL;Kästele V;Cerovic V;Cunningham AF;Henderson IR;Wall DM;Milling SWF
沙门氏菌感染是胃肠炎和全身性疾病的全球重要原因，是研究肠道免疫反应的有用工具。尽管对沙门氏菌的免疫应答机制已经进行了广泛的研究，但关于细菌如何从肠粘膜传播到肠系膜淋巴结(MLN)的问题仍然存在，MLN是抗原提呈的关键部位。在这项研究中，我们使用了一种小鼠感染沙门氏菌伤寒沙门氏菌(STM)的模型来确定在感染早期诱导的肠道免疫细胞的变化。然后，我们使用荧光标记的STM来鉴定通过淋巴迁移到MLN与感染部位的免疫细胞的相互作用。我们发现，存活的STM可以由迁移的树突状细胞的任何亚群在淋巴中携带，但不能由巨噬细胞携带。此外，淋巴中约有一半的STM根本与细胞无关，并自主移动。在MLN内，STM与树突状细胞和B细胞结合，但主要与MLN驻留的巨噬细胞结合。总而言之，我们描述了STM在感染后立即系统传播的途径。这种对感染过程的深入了解可能会为控制感染开辟新的途径。
Salmonella infection is a globally important cause of gastroenteritis and systemic disease and is a useful tool to study immune responses in the intestine. Although mechanisms leading to immune responses against Salmonella have been extensively studied, questions remain about how bacteria travel from the intestinal mucosa to the mesenteric lymph nodes (MLN), a key site for Ag presentation. In this study, we used a mouse model of infection with Salmonella enterica serovar Typhimurium (STM) to identify changes in intestinal immune cells induced during early infection. We then used fluorescently labeled STM to identify interactions with immune cells from the site of infection through migration in lymph to the MLN. We show that viable STM can be carried in the lymph by any subset of migrating dendritic cells but not by macrophages. Moreover, approximately half of the STM in lymph are not associated with cells at all and travel autonomously. Within the MLN, STM associates with dendritic cells and B cells but predominantly with MLN-resident macrophages. In conclusion, we describe the routes used by STM to spread systemically in the period immediately postinfection. This deeper understanding of the infection process could open new avenues for controlling it.
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鼠伤寒沙门氏菌通过穿透和破坏佩耶斑块的专门上皮细胞来引起鼠的感染。
影响因子：
15.3
作者：
Jones, Bradley D.;Ghori, Nafisa;Falkow, Stanley
通讯作者：
Falkow, Stanley
影响因子：
168.9
作者：
Feasey, Nicholas A.;Dougan, Gordon;Kingsley, Robert A.;Heyderman, Robert S.;Gordon, Melita A.
通讯作者：
Gordon, Melita A.
影响因子：
16.6
作者：
Mayer JU;Demiri M;Agace WW;MacDonald AS;Svensson-Frej M;Milling SW
通讯作者：
Milling SW
DOI：
10.1084/jem.177.5.1299
发表时间：
1993-05-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Liu LM;MacPherson GG
通讯作者：
MacPherson GG
影响因子：
56.9
作者：
Spadoni, Ilaria;Zagato, Elena;Rescigno, Maria
通讯作者：
Rescigno, Maria