Salmonella typhimurium initiates murine infection by penetrating and destroying the specialized epithelial M cells of the Peyer's patches.
Salmonella typhimurium initiates murine infection by penetrating and destroying the specialized epithelial M cells of the Peyer's patches.
复制标题
鼠伤寒沙门氏菌通过穿透和破坏佩耶斑块的专门上皮细胞来引起鼠的感染。
DOI：
10.1084/jem.180.1.15
复制
发表时间：
1994-07-01
影响因子：
15.3
通讯作者：
Falkow, Stanley
中科院分区：
文献类型：
--
作者：
Jones, Bradley D.;Ghori, Nafisa;Falkow, Stanley
已知沙门氏菌属物种通过穿透小肠的肠上皮来引发哺乳动物宿主的感染。这些细菌优先与派尔集合淋巴结相互作用，派尔集合淋巴结是构成肠道相关淋巴组织的淋巴滤泡的集合。我们感染小鼠结扎肠袢与侵入性和非侵入性鼠伤寒沙门氏菌菌株为30，60，120，和180分钟，并通过透射电子显微镜检查感染的组织。在30 min内，我们发现侵袭性S.鼠伤寒沙门氏菌专门进入派伊尔集合淋巴结的滤泡相关上皮（FAE）内发现的M细胞。最初，没有发现侵袭性细菌与邻近M细胞的肠上皮细胞之间的相互作用。M细胞的侵袭与细菌侵袭组织培养细胞的能力有关。S.鼠伤寒沙门氏菌突变体对组织培养细胞无侵袭性，在与M细胞或肠上皮细胞相关的连接环中孵育30、60、120或180 min后均未发现。鼠伤寒沙门氏菌对M细胞具有细胞毒性。M细胞的破坏在FAE中形成了一个缺口，使生物体能够侵入死细胞附近的肠上皮细胞。后来在感染过程中（120和180分钟），细菌的存在下的FAE与淋巴滤泡的细胞结构的变化。此外，复制沙门氏菌开始进入邻近感染的M细胞的肠上皮细胞的顶面和底侧面。
Salmonella species are known to initiate infection of mammalian hosts by penetrating the intestinal epithelium of the small bowel. These bacteria preferentially interact with Peyer's patches which are collections of lymphoid follicles making up the gut-associated lymphoid tissue. We infected murine ligated intestinal loops with invasive and noninvasive Salmonella typhimurium strains for 30, 60, 120, and 180 min and examined the infected tissue by transmission electron microscopy. Within 30 min, we found that invasive S. typhimurium exclusively entered M cells found within the follicle-associated epithelium (FAE) of the Peyer's patches. Initially, interactions between invasive bacteria and enterocytes adjacent to the M cells were not found. Invasion of M cells was associated with the ability of the bacteria to invade tissue culture cells. S. typhimurium mutants, which were noninvasive for tissue culture cells, could not be found in ligated loops associated with M cells or enterocytes after incubations of 30, 60, 120, or 180 min. At 60 min, internalized invasive S. typhimurium were cytotoxic for the M cells. Destruction of an M cell formed a gap in the FAE which allowed organisms to invade enterocytes adjacent to the dead cell. Later in the infection process (120 and 180 min), the presence of bacteria beneath the FAE correlated with changes in the cytoarchitecture of the lymphoid follicle. In addition, replicating Salmonella began to enter both the apical and basolateral surfaces of enterocytes adjacent to infected M cells.
登录
查看更多内容
DOI：
10.1073/pnas.89.13.5976
发表时间：
1992-07-01
影响因子：
11.1
作者：
GINOCCHIO, C;PACE, J;GALAN, JE
通讯作者：
GALAN, JE
影响因子：
6.4
作者：
OWEN, RL;PIERCE, NF;CRAY, WC
通讯作者：
CRAY, WC
DOI：
10.1084/jem.139.5.1189
发表时间：
1974-05-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Carter PB;Collins FM
通讯作者：
Collins FM
影响因子：
3.6
作者：
FRANCIS, CL;STARNBACH, MN;FALKOW, S
通讯作者：
FALKOW, S
影响因子：
64.8
作者：
GALAN, JE;PACE, J;HAYMAN, MJ
通讯作者：
HAYMAN, MJ